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Planta Med 2010; 76(10): 998-1003
DOI: 10.1055/s-0029-1240869
Natural Product Chemistry
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

New Secoiridoid Glucosides from Ligustrum lucidum Induce ERK and CREB Phosphorylation in Cultured Cortical Neurons

Guangmiao Fu1 , Fanny C. F. Ip1 , Haihong Pang1 , Nancy Y. Ip1
  • 1Department of Biochemistry, Molecular Neuroscience Center, and Biotechnology Research Institute, The Hong Kong University of Science and Technology, Hong Kong SAR, China
Further Information

Publication History

received July 4, 2009 revised January 12, 2010

accepted January 16, 2010

Publication Date:
08 February 2010 (online)

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Abstract

Two new secoiridoid glucosides, namely iso-oleonuezhenide (1) and methyloleoside 7-ethyl ester (2), along with five known ones, oleonuezhenide (3), nuezhenide (4), oleuropein (5), G13 (6), and jaspolyside methyl ester (7), were isolated from the fruits of Ligustrum lucidum. Their structures were assigned based on 1H‐NMR, 13C‐NMR, and 2D‐NMR analyses, in combination with HR‐MS experiments and the comparison with literature data of related compounds, as well as on chemical experiments. We have examined the ability of these compounds to activate ERK and CREB in cultured cortical neurons. Our studies demonstrate that compound 1 induces ERK and CREB phosphorylation in primary cortical neurons in a dose- and temporal-dependent manner, suggesting its bioactivity on neurons.

Key words

Ligustrum lucidum - Oleaceae - secoiridoid glucosides - iso‐oleonuezhenide - methyloleoside 7‐ethyl ester - ERK - CREB

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Prof. Nancy Y. Ip

Department of Biochemistry
The Hong Kong University of Science and Technology

Clear Water Bay

Kowloon, Hong Kong SAR

China

Phone: + 85 2 23 58 72 89

Fax: + 85 2 23 58 27 65

Email: boip@ust.hk

    www.thieme-connect.de/ejournals/toc/plantamedica