Planta Med 2010; 76(16): 1852-1858
DOI: 10.1055/s-0030-1249971
Pharmacology
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Larvicidal Constituents of Zingiber officinale (Ginger) against Anisakis simplex

Rong-Jyh Lin1 , 2 , Chung-Yi Chen3 , June-Der Lee1 , Chin-Mei Lu1 , 4 , Li-Yu Chung1 , Chuan-Min Yen1
  • 1Department of Parasitology and Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
  • 2School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan
  • 3School of Medical and Health Sciences, Fooyin University, Kaohsiung County, Taiwan
  • 4Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan
Further Information

Publication History

received February 3, 2010 revised April 17, 2010

accepted April 23, 2010

Publication Date:
08 June 2010 (online)

Abstract

In this study, we investigated the anthelmintic activity of [10]-shogaol, [6]-shogaol, [10]-gingerol and [6]-gingerol, compounds isolated from the roots of Zingiber officinale L., Zingiberaceae (ginger), against Anisakis simplex. The above compounds kill or reduce spontaneous movement in A. simplex larvae. The maximum lethal efficacy of [10]-shogaol and [10]-gingerol was approximately 80 % and 100 %, respectively. We further examined the time course of compound-induced loss of mobility in A. simplex. The results showed that various concentrations of [10]-shogaol, [6]-shogaol, [10]-gingerol and [6]-gingerol have maximum effects on loss of spontaneous movement from 24 to 72 h. In addition, the time course of mortality and the percentage of loss of spontaneous movements were ascertained to determine the minimum effective doses of [10]-gingerol and [10]-shogaol. [10]-Gingerol exhibited a larger maximum larvicidal effect and greater loss of spontaneous movement than [10]-shogaol and albendazole. In addition, these constituents of Zingiber officinale showed effects against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and peroxyl radicals. These constituents of Zingiber officinale are responsible for its larvicidal activity against A. simplex.

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Chuan-Min Yen

Department of Parasitology and Institute of Medicine
College of Medicine
Kaohsiung Medical University

100 shih-Chuan 1st Road

Kaohsiung, 80708

Taiwan

Republic of China

Phone: + 8 86 73 12 11 01 ext. 21 69

Fax: + 8 86 73 21 83 09

Email: chmiye@kmu.edu.tw