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Planta Med 2010; 76(16): 1927-1933
DOI: 10.1055/s-0030-1250063
Biochemistry, Molecular Biology and Biotechnology
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Analysis of the Genetic Diversity and Affinities of Different Iranian Satureja Species Based on SAMPL Markers

Javad Hadian1 , Ali Azizi2 , Mohammad Fakhr Tabatabaei3 , Mohammad Reza Naghavi4 , Ziba Jamzad5 , Wolfgang Friedt2
  • 1Medicinal Plants and Drug Research Institute, Shahid Beheshti University, G. C., Evin, Tehran, Iran
  • 2Department of Crop Sciences and Plant Breeding I, Justus-Liebig University Giessen, Giessen, Germany
  • 3Department of Horticultural Sciences, Faculty of Agriculture, University of Tehran, Karaj, Iran
  • 4Department of Plant Breeding, Faculty of Agriculture, University of Tehran, Karaj, Iran
  • 5Research Institute of Forests and Rangelands, Tehran, Iran
Further Information

Publication History

received Nov. 14, 2009 revised May 26, 2010

accepted June 1, 2010

Publication Date:
01 July 2010 (online)

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Abstract

The different species of the genus Satureja are known as “Marze Kohi” in Iran. Herbal drugs of these plants have long been used in traditional medicine, and some species are currently used in food and pharmaceutical industries. In the present work, the genetic diversity and affinities of several Satureja species were studied based on Selectively Amplified Microsatellite Polymorphic Loci (SAMPL) using five primer combinations. A total of 515 polymorphic DNA fragments were amplified, with a mean of 103 bands per assay. Among the species the maximum distance was observed between S. hortensis and S. isophylla, while the maximum degree of similarity was obtained between S. rechingeri and S. khuzistanica. Cluster analysis was performed for all SAMPL data using the neighbor-joining method. All species for which multiple accessions were sampled were monophyletic with maximum bootstrap support. The investigated species were clustered in three main groups. In general, the relationships among Satureja species revealed by the cluster analysis were somewhat congruent with their geographical distributions.

Key words

Satureja spp - Lamiaceae - genetic affinities - SAMPL markers

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Javad Hadian

Medicinal Plants and Drug Research Institute
Shahid Beheshti University, G. C., Evin

Chamran Street

19835-389 Tehran

Iran

Phone: + 98 21 22 43 17 83

Fax: + 98 21 22 43 17 83

Email: j_hadian@sbu.ac.ir

    www.thieme-connect.de/ejournals/toc/plantamedica