1Department of Biochemistry and Molecular Biology, Aging-associated Vascular Disease Research Center, College of Medicine, Yeungnam University, Daegu, Republic of Korea
2College of Pharmacy, Yeungnam University, Gyongsan, Republic of Korea
3Department of Laboratory Medicine, CHA Gumi Medical Center, CHA University, Gyeongsangbuk-do, Republic of Korea
4College of Pharmacy, Yanbian University, Yanji, P. R. China
Since cellular senescence involves organismal aging as well as diverse diseases, aging intervention might contribute to inhibit the aging process as well as aging-associated diseases. We tried to search for effective compounds from the root bark of Ulmus davidiana that are able to inhibit cellular senescence in human fibroblasts (HDFs) and human umbilical vein endothelial cells (HUVECs). Twenty-two compounds from the root bark of U. davidiana were isolated and screened for their inhibitory effects on adriamycin-induced cellular senescence by measuring senescence-associated β-galatosidase (SA-β-gal) activity. Among twenty-two compounds isolated, epifriedelanol (3), ssioriside (15), and catechin-7-O-β-D-glucopyranoside (22) had inhibitory effects on adriamycin-induced cellular senescence in HDFs. Friedelin (2), epifriedelanol (3), and catechin-7-O-β-apiofuranoside (18) were active in HUVECs. In particular, epifriedelanol (3) suppressed adriamycin-induced cellular senescence as well as replicative senescence in HDFs and HUVECs. These results suggest that epifriedelanol (3) reduces cellular senescence in human primary cells and might be used to develop dietary supplements or cosmetics that modulate tissue aging or aging-associated diseases.
Key words
Ulmus davidiana var. japonica
- Ulmaceae - cellular senescence - epifriedelanol - human primary cells - aging intervention
Supporting Information for this article is available online at
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