Abstract
A new diarylheptanoid, juglanin C (1), was isolated from the 80 % methanolic extract of the leaves and twigs of Juglans sinensis with three known diarylheptanoids, juglanin A (2), juglanin B (3), and (5R)-5-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1(4-hydroxyphenyl)-3-heptanone
(4), using bioactivity-guided fractionation and chromatographic techniques. Among the
isolated diarylheptanoids, compounds 1 and 2 significantly showed neuroprotective activities against glutamate-induced toxicity
in HT22 cells. These two diarylheptanoids significantly reduced the overproduction
of cellular peroxide in glutamate-injured HT22 cells. Moreover, these two diarylheptanoids
significantly maintained antioxidative defense systems, including glutathione, glutathione
reductase, and glutathione peroxidase, under glutamate-induced oxidative stress in
HT22 cells.
Key words
Juglans sinensis
- Juglandaceae - diarylheptanoid - HT22 hippocampal cells - antioxidant activity -
neuroprotective
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Prof. PhD Young Choong Kim
College of Pharmacy and Research Institute of Pharmaceutical Sciences
Seoul National University
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