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DOI: 10.1055/s-0030-1255021
© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York
Low-Carbohydrate and High-Fat Diets on the Promotion of Hepatic Steatosis in Rats
Publikationsverlauf
received 25.01.2010
first decision 06.04.2010
accepted 11.05.2010
Publikationsdatum:
08. Juni 2010 (online)

Abstract
Aim: The present work looked for to evaluate in rats the impact of different diets (high-lipid and high-lipid+high-protein) on liver, verifying the occurrence of oxidative stress and steatosis.
Methods: The animals were treated with the respective diets (Group HLS: high-lipid diet with 50% of saturated fat; Group HPLS: high-lipid and high-protein diet with 50% of saturated fat and 40% of protein; Group Control: control diet AIN-93) for 28 days. After this period the animals were sacrificed for hepatic determinations of MDA, reduced GSH, vitamin E, steatosis and glycemia.
Results: The results showed higher glycemia in the group HPLS, high concentration of MDA and GSH in the group Control and decreased hepatic vitamin E concentration in the groups that received the high-lipid diets. The hepatic fat was higher in the groups HPLS and HLS in relation to the Group Control, however HPLS presenting high level of fat concentration, showing similar results as the steatosis.
Conclusion: the fat increase in the diet promoted increase of the oxidative stress, evidenced by the decrease in the hepatic concentration of vitamin E, showing its antioxidant role against the probable generated free radicals, the ones which possibly exercised a role in the steatosis occurrence.
Key words
hepatic steatosis - high-protein diet - high-fat diet
References
- 1
Anstee QM, Goldin RD.
Mouse models in non-alcoholic fatty liver disease and steatohepatitis research.
International Journal of Experimental Pathology.
2006;
87
1-16
MissingFormLabel
- 2
Arnaud J, Fortis I, Blachier S. et al .
Simultaneous determination of retinol, alpha-tocopherol and beta-carotene in serum
by isocratic high-performance liquid chromatography.
Journal of chromatography.
1991;
6; 572
103-116
MissingFormLabel
- 3
Axen KV, Dikeakos A, Sclafani A.
High dietary fat promotes syndrome X in nonobese rats.
J. Nutri.
2003;
133
2244-2249
MissingFormLabel
- 4
Birkner E, Kasperczyk S, Kasperczyk A. et al .
Metabolic and antioxidative changes in liver steatosis induced by high-fat, low-carbohydrate
diet in rabbits.
J Physiol Pharmacol.
2005;
56
(S 06)
45-58
MissingFormLabel
- 5
Bligh EG, Dyer WJ.
A rapid method of total lipid extraction and purification.
Canadian journal of biochemistry and physiology.
1959;
37
911-917
MissingFormLabel
- 6
Brehm BJ, Seeley RJ, Daniels SR. et al .
A randomized trial comparing a very low carbohydrate diet and a calorie-restricted
low fat diet on body weight and cardiovascular risk factors in healthy women.
The Journal of Clinical Endocrinology and Metabolism.
2003;
88
1617-1623
MissingFormLabel
- 7
Brigelius-Flohe R, Kelly FJ, Salonen JT. et al .
The European perspective on vitamin E: current knowledge and future research.
The American journal of clinical nutrition.
2002;
76
703-716
MissingFormLabel
- 8
Browning JD, Horton JD.
Molecular mediators of hepatic steatosis and liver injury.
The Journal of clinical investigation.
2004;
114
147-152
MissingFormLabel
- 9
Buege JA, Aust SD.
Microsomal lipid peroxidation.
Methods in enzymology.
1978;
52
302-310
MissingFormLabel
- 10
Das SK, Balakrishnan V, Mukherjee S. et al .
Evaluation of blood oxidative stress-related parameters in alcoholic liver disease
and non-alcoholic fatty liver disease.
Informa Healthcare.
2008;
323-334
MissingFormLabel
- 11
Dowman JK, Tomlinson JW, Newsome PN.
Pathogenesis of non-alcoholic fatty liver disease.
QJ Med.
2010;
103
71-83
MissingFormLabel
- 12
Del Rio D, Stewart AJ, Pellegrini N.
A review of recent studies on malondialdehyde as toxic molecule and biological marker
of oxidative stress.
Nutrition, Metabolism and Cardiovascular Diseases.
2005;
15
316-328
MissingFormLabel
- 13
Fan JG, Qiao L.
Commonly used animal models of non-alcoholic steatohepatitis.
Hepatobiliary Pancreat Dis Int.
2009;
8
233-240
MissingFormLabel
- 14
Feldstein AE, Canbay A, Guicciardi ME. et al .
Diet associated hepatic steatosis sensitizes to Fas mediated liver injury in mice.
Journal of hepatology.
2003;
39
978-983
MissingFormLabel
- 15
Franco R, Schoneveld OJ, Pappa A. et al .
The central role of glutathione in the pathophysiology of human diseases.
Archives of physiology and biochemistry.
2007;
113
234-258
MissingFormLabel
- 16
Grattagliano I, Vendemiale G, Caraceni P. et al .
Starvation impairs antioxidant defense in fatty livers of rats fed a choline-deficient
diet.
The Journal of nutrition.
2000;
130
2131-2136
MissingFormLabel
- 17
Grattagliano I, Portincasa P, Palmieri VO. et al .
Managing nonalcoholic fatty liver disease: Recommendations for family physicians.
Can Fam Physician.
2007;
53
857-863
MissingFormLabel
- 18
Hammond KA, Janes DN.
The effects of increased protein intake on kidney size and function.
J Exp Biol.
1998;
201
2081-2090
MissingFormLabel
- 19
Kashireddy PV, Rao MS.
Lack of peroxisome proliferator-activated receptor alpha in mice enhances methionine
and choline deficient diet-induced steatohepatitis.
Hepatol Res.
2004;
30
104-110
MissingFormLabel
- 20
Koppe SW, Sahai A, Malladi P. et al .
Pentoxifylline attenuates steatohepatitis induced by the methionine choline deficient
diet.
Journal of hepatology.
2004;
41
592-598
MissingFormLabel
- 21
Koteish A, Diehl AM.
Animal models of steatosis.
Seminars in liver disease.
2001;
21
89-104
MissingFormLabel
- 22
Lacroix M, Gaudichon C, Martin A. et al .
A long-term high-protein diet markedly reduces adipose tissue without major side effects
in Wistar male rats.
Am J Physiol Regul Integr Comp Physiol.
2004;
287
R934-R942
MissingFormLabel
- 23
Larter CZ, Yeh MM.
Animal models of NASH: Getting both pathology and metabolic context right.
Journal of Gastroenterology and Hepatology.
2008;
23
1635-1648
MissingFormLabel
- 24
Lieber CS, Leo MA, Mak KM. et al .
Model of nonalcoholic steatohepatitis.
The American journal of clinical nutrition.
2004;
79
502-509
MissingFormLabel
- 25
Linn T, Santosa B, Gronemeyer D. et al .
Effect of long-term dietary protein intake on glucose metabolism in humans.
Diabetologia.
2000;
43
1257-1265
MissingFormLabel
- 26
Oh SI, Kim CI, Chun HJ. et al .
Chronic ethanol consumption affects glutathione status in rat liver.
The Journal of nutrition.
1998;
128
758-763
MissingFormLabel
- 27
Oliveira CP, Coelho AM, Barbeiro HV. et al .
Liver mitochondrial dysfunction and oxidative stress in the pathogenesis of experimental
nonalcoholic fatty liver disease.
Brazilian journal of medical and biological research.
2006;
39
189-194
MissingFormLabel
- 28
Petzke KJ, Elsner A, Proll J. et al .
Long-term high protein intake does not increase oxidative stress in rats.
J Nutr.
2000;
130
2889-2896
MissingFormLabel
- 29
Pichon L, Huneau JF, Fromentin G. et al .
A high-protein, high-fat, carbohydrate-free diet reduces energy intake, hepatic lipogenesis,
and adiposity in rats.
The Journal of nutrition.
2006;
136
1256-1260
MissingFormLabel
- 30
Reeves PG, Nielsen FH, Fahey Jr GC.
AIN-93 purified diets for laboratory rodents: final report of the American Institute
of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet.
The Journal of nutrition.
1993;
123
1939-1951
MissingFormLabel
- 31
Reis KAA, Roquete MLV, Penna FJ.
Esteatohepatite não-alcoólica na infância e adolescência: revisão.
Pediatria (São Paulo).
2001;
23
329-339
MissingFormLabel
- 32
Scheuer PJ.
The morphology of alcoholic liver disease.
British Medical Bulletin.
1982;
38
63-65
MissingFormLabel
- 33
Sedlak J, Lindsay RH.
Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with
Ellman's reagent.
Analytical Biochemistry.
1968;
24, 25
192-205
MissingFormLabel
- 34
Traber MG.
Vitamin E regulatory mechanisms.
Annual review of nutrition.
2007;
27
347-362
MissingFormLabel
- 35
Zeisel SH, Da Costa KA, Franklin PD. et al .
Choline, an essential nutrient for humans.
Faseb J.
1991;
5
2093-2098
MissingFormLabel
- 36
Zivkovic AM, German JB, Sanyal AJ.
Comparative review of diets for the metabolic syndrome: implications for nonalcoholic
fatty liver disease.
Am J Clin Nutr.
2007;
86
285-300
MissingFormLabel
- 37
Zou Y, Li J, Lu C. et al .
High-fat emulsion-induced rat model of nonalcoholic steatohepatitis.
Life Sciences.
2006;
79
1100-1107
MissingFormLabel
Correspondence
D. S. Leonardi
Departamento de Patologia da
Faculdade de Medicina de
Ribeirão Preto
Universidade de São Paulo
Av Bandeirantes 3900 SP
14049-900 Ribeirão Preto
Brasil
eMail: dsleonardi@gmail.com