Stem Cell and Peripheral Nerve Injury and Repair

ABSTRACT

Peripheral motor nerve injuries are a significant source of morbidity. Neural stem cells (NSCs), a group of relatively primitive cells, possess self-renewal ability and multidifferentiation potential. NSCs may be successfully separated from the human embryo and central nervous system (CNS) and differentiated into mature neurons and gliacytes by in vitro induction or transplantation into the body and may be differentiated into Schwann-like cells under specific conditions. It has been demonstrated that the ability of peripheral nerves to regenerate is mainly attributable to Schwann cells. NSC transplantation can promote peripheral nerve regeneration and provide a new means for treatment of peripheral nerve injury. In recent years, the study of NSCs has become a focus of many laboratories, but the biological characteristics and differentiation regulation mechanisms are not fully clear. In this article, we provide a brief review of NSC characteristics, cultivation, oriented differentiation, and clinical application.

KEYWORDS

Neural stem cell - transplantation - peripheral nerve

REFERENCES

• 1 Parker M A, Anderson J K, Corliss D A et al.. Expression profile of an operationally-defined neural stem cell clone.  Exp Neurol. 2005;  194 320-332
• 2 Wen T, Li H, Song H et al.. Down-regulation of specific gene expression by double-strand RNA induces neural stem cell differentiation in vitro.  Mol Cell Biochem. 2005;  275 215-221
• 3 Svendsen C N, Skepper J, Rosser A E, ter Borg M G, Tyres P, Ryken T. Restricted growth potential of rat neural precursors as compared to mouse.  Brain Res Dev Brain Res. 1997;  99 253-258
• 4 Zheng X S, Yang X F, Liu W G, Shen G, Pan D S, Luo M. A novel method for culturing neural stem cells.  In Vitro Cell Dev Biol Anim. 2007;  43 155-158
• 5 Zhou Y F, Fang F, Fu J R et al.. An experimental study on astrocytes promoting production of neural stem cells derived from mouse embryonic stem cells.  Chin Med J (Engl). 2005;  118 1994-1999
• 6 Villa A, Snyder E Y, Vescovi A, Martínez-Serrano A. Establishment and properties of a growth factor-dependent, perpetual neural stem cell line from the human CNS.  Exp Neurol. 2000;  161 67-84
• 7 Martin D, Schoenen J, Delrée P, Leprince P, Rogister B, Moonen G. Grafts of syngenic cultured, adult dorsal root ganglion-derived Schwann cells to the injured spinal cord of adult rats: preliminary morphological studies.  Neurosci Lett. 1991;  124 44-48
• 8 Sulaiman O A, Gordon T. Effects of short- and long-term Schwann cell denervation on peripheral nerve regeneration, myelination, and size.  Glia. 2000;  32 234-246
• 9 Goldman S A, Sim F. Neural progenitor cells of the adult brain.  Novartis Found Symp. 2005;  265 66-80; discussion 82–97
• 10 Filip S, Mokrý J, Karbanová J et al.. The transplantation of neural stem cells and predictive factors in hematopoietic recovery in irradiated mice.  Transfus Apheresis Sci. 2005;  32 157-166
• 11 Blakemore W F. The case for a central nervous system (CNS) origin for the Schwann cells that remyelinate CNS axons following concurrent loss of oligodendrocytes and astrocytes.  Neuropathol Appl Neurobiol. 2005;  31 1-10
• 12 Heath C A. Cells for tissue engineering.  Trends Biotechnol. 2000;  18 17-19
• 13 Batts S A, Raphael Y. Transdifferentiation and its applicability for inner ear therapy.  Hear Res. 2007;  227 41-47
• 14 Fritzsch B, Beisel K W, Hansen L A. The molecular basis of neurosensory cell formation in ear development: a blueprint for hair cell and sensory neuron regeneration?.  Bioessays. 2006;  28 1181-1193
• 15 Matsuoka A J, Kondo T, Miyamoto R T, Hashino E. Enhanced survival of bone-marrow-derived pluripotent stem cells in an animal model of auditory neuropathy.  Laryngoscope. 2007;  117 1629-1635
• 16 Naito Y, Nakamura T, Nakagawa T et al.. Transplantation of bone marrow stromal cells into the cochlea of chinchillas.  Neuroreport. 2004;  15 1-4
• 17 Hu Z, Wei D, Johansson C B et al.. Survival and neural differentiation of adult neural stem cells transplanted into the mature inner ear.  Exp Cell Res. 2005;  302 40-47
• 18 Hu Z Q, Ulfendahl M, Olivius N P. Central migration of neuronal tissue and embryonic stem cells following transplantation along the adult auditory nerve.  Brain Res. 2004;  1026 68-73
• 19 Ernfors P, Duan M L, ElShamy W M, Canlon B. Protection of auditory neurons from aminoglycoside toxicity by neurotrophin-3.  Nat Med. 1996;  2 463-467
• 20 Duan M L, Agerman K, Ernfors P, Canlon B. Complementary roles of neurotrophin-3 and NMDA antagonist in protecting against noise and aminoglycoside-induced ototoxicity.  Proc Natl Acad Sci U S A. 2000;  97 7597-7602
• 21 Iguchi F, Nakagawa T, Tateya I et al.. Trophic support of mouse inner ear by neural stem cell transplantation.  Neuroreport. 2003;  14 77-80
• 22 Chen Q, Wei-wei G, Wu Y et al.. Adenovirus-mediated NT3 gene transfer protects spiral ganglion neurons from degeneration after noise trauma.  Sheng Li Xue Bao. 2002;  54 263-266
• 23 Donzelli R, Maiuri F, Peca C, Cavallo L M, Motta G, de Divitiis E. Microsurgical repair of the facial nerve.  Zentralbl Neurochir. 2005;  66 63-69
• 24 Malik T H, Kelly G, Ahmed A, Saeed S R, Ramsden R T. A comparison of surgical techniques used in dynamic reanimation of the paralyzed face.  Otol Neurotol. 2005;  26 284-291
• 25 Sinis N, Schaller H E, Schulte-Eversum C et al.. Nerve regeneration across a 2-cm gap in the rat median nerve using a resorbable nerve conduit filled with Schwann cells.  J Neurosurg. 2005;  103 1067-1076
• 26 Chang-xiang Y, Yi-hua A, Jun-hua L, Shu-ling L, Hong W, Chun-jiang Y. Research on repairing facial nerve injury of rabbits by neural stem cells and autologous fasia.  Chinese Journal of Rehabilitation Theory and Practice. 2004;  10 21-22
• 27 Guo B, Dong M, Ren X. Research of guinea pig neural stem cells promoting facial nerve regeneration in rabbits.  Journal of Zhengzhou University (Medical Science). 2006;  41(3) 445-448
• 28 Li Z, Liu L, Tian W, Teng G, Zhang Y, Wang F. MR tracking of transplanted neural stem cells labeled with SPIO nanoparticles in rata brain after facial nerve injury.  Chin J Med Imaging Technol. 2009;  25(11) 1941-1944
• 29 Ventadour S, Attaix D. Mechanisms of skeletal muscle atrophy.  Curr Opin Rheumatol. 2006;  18 631-635
• 30 Burns A S, Boyce V S, Tessler A, Lemay M A. Fibrillation potentials following spinal cord injury: improvement with neurotrophins and exercise.  Muscle Nerve. 2007;  35 607-613
• 31 Yun-dong S, Jian-guang X, Xu W, Xu L, Lu J, Gu Y. Experimental study on neural stem cell transplantation delaying denervated muscle atrophy.  Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2008;  22 1051-1055
• 32 Wei P, Liu J, Zhou H L et al.. Effects of engrafted neural stem cells derived from GFP transgenic mice in Parkinson's diseases rats.  Neurosci Lett. 2007;  419 49-54
• 33 Dezawa M, Takahashi I, Esaki M, Takano M, Sawada H. Sciatic nerve regeneration in rats induced by transplantation of in vitro differentiated bone-marrow stromal cells.  Eur J Neurosci. 2001;  14 1771-1776
• 34 Murakami T, Fujimoto Y, Yasunaga Y et al.. Transplanted neuronal progenitor cells in a peripheral nerve gap promote nerve repair.  Brain Res. 2003;  974 17-24
• 35 MacDonald S C, Fleetwood I G, Hochman S et al.. Functional motor neurons differentiating from mouse multipotent spinal cord precursor cells in culture and after transplantation into transected sciatic nerve.  J Neurosurg. 2003;  98 1094-1103
• 36 Almond S, Lindley R M, Kenny S E, Connell M G, Edgar D H. Characterization and transplantation of enteric nervous system progenitor cells.  Gut. 2007;  56 489-496
• 37 Zhong-rong L, Li-bin Z, Zheng-ji L, Li D, Cheng-ren S. Primary study of homotransplantations of enteric neural stem cells into the alimentary tract in rat.  Chinese Journal of Pediatric Surgery. 2007;  28 366-369
• 38 Micci M A, Kahrig K M, Simmons R S, Sarna S K, Espejo-Navarro M R, Pasricha P J. Neural stem cell transplantation in the stomach rescues gastric function in neuronal nitric oxide synthase-deficient mice.  Gastroenterology. 2005;  129 1817-1824
• 39 Xian-qing J, Ji-rong X, Xiao-zhang C. A preliminary study on the treatment of experimental aganglionosis by mesenchymal stem cells transplantation into colon in vivo.  Chinese Journal of Pediatric Surgery. 2007;  28 481-485
• 40 Shimojo H, Ohtsuka T, Kageyama R. Oscillations in notch signaling regulate maintenance of neural progenitors.  Neuron. 2008;  58(1) 52-64