Maternal-Fetal Outcome, Lipid Profile and Oxidative Stress of Diabetic Rats Neonatally Exposed to Streptozotocin

D. C. Damasceno; A. C. I. Kiss; Y. K. Sinzato; K. E. de Campos; M. V. C. Rudge; I. M. P. Calderon; G. T. Volpato

doi:10.1055/s-0030-1269886

Experimental and Clinical Endocrinology & Diabetes, Inhaltsverzeichnis

Exp Clin Endocrinol Diabetes 2011; 119(7): 408-413
DOI: 10.1055/s-0030-1269886

Article

Maternal-Fetal Outcome, Lipid Profile and Oxidative Stress of Diabetic Rats Neonatally Exposed to Streptozotocin

D. C. Damasceno¹ , A. C. I. Kiss¹ , Y. K. Sinzato¹ , K. E. de Campos¹ , M. V. C. Rudge¹ , I. M. P. Calderon¹ , G. T. Volpato¹ ^, ²

¹Laboratory of Experimental Research of Gynecology and Obstetrics, Department of Gynecology and Obstetrics, Botucatu Medical School – São Paulo State University (Unesp), SP, Brazil.
²Institute of Biological and Health Sciences, University Center of Araguaia, Mato Grosso Federal University (UFMT), Barra do Garças, Mato Grosso, Brazil

Abstract

Background: There is no evidence about the integrated issue on glycemia, lipid profile, oxidative stress, and anomaly frequency of pregnant diabetic rats neonatally exposed to streptozotocin.

Objective: Evaluating the impact of hyperglycemia in diabetic rats neonatally exposed to streptozotocin on maternal reproductive and fetal outcomes and the relationship with lipid profile and maternal oxidative stress.

Material and Methods: Ten 90-day-old female Wistar rats were mated to obtain offspring. Some of these newborns received streptozotocin (70 mg/kg, i. p. – n5-STZ group) and the remainder given only citrate buffer (control group) on their day 5 of life. At adult life, these rats (n=13 animals/group) were mated and, at day 21 of pregnancy, they were killed to obtain a maternal blood samples for biochemical determinations. The gravid uterus was weighed with its contents and fetuses were analyzed.

Results: At day 0 of pregnancy, glycemic means of n5-STZ rats were significantly greater compared to those of control rats, but presented fetuses classified as small for pregnancy age. The n5-STZ rats showed increased total cholesterol, triglycerides, MDA concentrations, lower SOD activity and increased frequency fetal visceral anomalies as compared to the control group.

Conclusion: This study showed that the experimental model used led to mild hyperglycemia during pregnancy, although it did not lead to increased macrosomic fetus rates. The hyperglycemic maternal environment caused metabolic alterations, including increased triglyceride and total cholesterol concentrations, and elevated oxidative stress, contributing to increase fetal visceral anomalies.

Key words

mild diabetes - streptozotocin - pregnancy - oxidative stress - anomaly - rat

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References

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Correspondence

Profa. Dra. D. C. Damasceno

Departamento de Ginecologia

e Obstetrícia

Faculdade de Medicina de

Botucatu – Unesp

Distrito de Rubião Júnior s/n

CEP.18618-000 – BOTUCATU – SP

BRASIL

Telefon: +55/14/ 3811 6181

Fax: +55/14/ 3811 6181

eMail: damasceno@fmb.unesp.br