Fraxinus rhynchophylla showed significant inhibitory activity on adipocyte differentiation in the 3T3-L1 preadipocyte cell line as assessed by measuring fat accumulation using Oil Red O staining. Further fractionation led to the isolation of two secoiridoids, oleuropein and hydroxyframoside B. Hydroxyframoside B significantly reduced fat accumulation and triglyceride content in differentiated 3T3-L1 cells without affecting cell viability, whereas oleuropein showed little effect. Further studies with interval treatment demonstrated that hydroxyframoside B exerted inhibitory activity on adipocyte differentiation when treated within 2 days (days 0–2) after differentiation induction. In addition, hydroxyframoside B significantly blocked the induction of adipogenic transcription factors such as C/EBPα, C/EBPβ, and PPARγ. Taken together, these results suggest that hydroxyframoside B inhibited early/middle stage of adipogenic differentiation, in part, via inhibition of C/EBPα, C/EBPβ, and PPARγ-dependent pathways.
5
Park S H, Kim S H, Kim Y S, Ryu S Y, Hwang J T, Yang H J, Kim G H, Kwon D Y, Kim M S.
Luteolin inhibits adipogenic differentiation by regulating PPARγ activation.
Biofactors.
2009;
35
373-379
6
Moon H S, Chung C S, Lee H G, Kim T G, Choi Y J, Cho C S.
Inhibitory effect of (−)-epigallocatechin-3-gallate on lipid accumulation of 3T3-L1 cells.
Obesity.
2007;
15
2571-2582
7
Ahn J, Lee H, Kim S, Park J, Ha T.
The anti-obesity effect of quercetin is mediated by the AMPK and MAPK signaling pathways.
Biochem Biophys Res Commun.
2008;
373
545-549
8
Rayalam S, Yang J-Y, Ambati S, Della-Fera M A, Baile C A.
Resveratrol induces apoptosis and inhibits adipogenesis in 3T3-L1 adipocytes.
Phytother Res.
2008;
22
1367-1371
9
Shin E, Choi K-M, Yoo H-S, Lee C-K, Hwang B Y, Lee M K.
Inhibitory effects of coumarins from the stem barks of Fraxinus rhynchophylla on adipocyte differentiation in 3T3-L1 cells.
Biol Pharm Bull.
2010;
33
1610-1614
10
He Z D, But P P H, Chan T W D, Dong H, Xu H X, Lau C P, Sun H D.
Antioxidative glucosides from the fruits of Ligustrum lucidum.
Chem Pharm Bull.
2001;
49
780-784