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Planta Med 2011; 77(13): 1562-1566
DOI: 10.1055/s-0030-1270833
Analytical Studies
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Quantitative Determination of 10 Phenylpropanoid and Lignan Compounds in Lancea tibetica by High-Performance Liquid Chromatography with UV Detection

Zong-Hua Song1 , 2 , Yan-Hong Wang1 , Zhong-Zhi Qian2 , Troy J. Smillie1 , Ikhlas A. Khan1 , 3
  • 1National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, Mississippi, USA
  • 2Chinese Pharmacopeia Commission, Beijing, P. R. China
  • 3Department of Pharmacognosy, University of Mississippi, University, Mississippi, USA
Further Information

Publication History

received Sept. 28, 2010 revised January 24, 2011

accepted February 2, 2011

Publication Date:
23 February 2011 (online)

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Abstract

An HPLC method was developed for simultaneous determination of one phenylpropanoid glycoside, verbascoside (1), and nine lignans, including lantibeside (2), phillyrin (3), lantibeside B (4), lantibeside C (5), tibeticoside A (6), styraxjaponoside C (7), sylvatesmin (8), (+)-piperitol (9), and horsfieldin (10), from the Tibetan medicinal plant Lancea tibetica Hook. F. et Thoms. The analysis was performed within 45 min. The extraction method was optimized with different solvent systems. The HPLC method was validated for linearity, repeatability, accuracy, limits of detection, and limits of quantification. The limits of detection and limits of quantification of 10 analytes were found to be less than 0.1 and 0.5 µg/mL, respectively. The RSD for intra- and inter-day analyses was less than 4.2 %, and the recovery efficiency was 90–105 %. The method was used to analyze different populations of L. tibetica collected in China. HPLC profiles showed that the concentrations of analytes were different in samples collected from different areas of China. Verbascoside was the dominant component in three out of five plant samples; compounds 2, 3, 6, and 8 accounted for over 62 % yields in total lignan contents. The method is useful for identification, quality assurance, and quality control of L. tibetica and its related products.

Key words

Lancea tibetica - Scrophulariaceae - HPLC‐UV - verbascoside - lignan

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Prof. Dr. Ikhlas A. Khan

National Center for Natural Products Research
School of Pharmacy
University of Mississippi

University, MS 38677

USA

Phone: +1 66 29 15 78 21

Fax: +1 66 29 15 79 89

Email: ikhan@olemiss.edu