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Exp Clin Endocrinol Diabetes 2011; 119(5): 265-270
DOI: 10.1055/s-0031-1271705
Article

© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Proteome Analysis of Differential Protein Expression in Brain of Rats with Type 1 Diabetes Mellitus

X. Li1 , W. Pan1 , G. Z. Yang1 , Y. N. Di1 , F. Zhao1 , L. Y. Zhu1 , Z. H. Jiang1
  • 1Department of Medical Laboratory, Guiyang Medical College, Department of Clinical Biochemistry, Affiliated Hospital of Guiyang Medical College, Guiyang, Guizhou, P.R. China
Further Information

Publication History

received 26.11.2010 first decision 15.01.2011

accepted 19.01.2011

Publication Date:
06 April 2011 (online)

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Abstract

Aim of the study: Proteomic approach was applied to identify differential protein expressed in brain of rats with type 1 diabetes mellitus (T1DM) in order to search for potential biomarkers for pathological changes of brain with T1DM.

Methods: Proteins were extracted from brain tissues of T1DM rats and healthy control rats, separated by 2-DE and identified by MALDI-TOF-MS. The results were validated by Western blot analysis and immunohistochemistry (IHC).

Results: A total of 8 proteins from the 24 differentially expressed spots were identified by MALDI-TOF-MS. The proteins identified were vitamin D-dependent calcium-binding protein, creatine kinase B-type (B-CK), myosin light chain kinase (MLCK), HSP60 and HSP71, ATP synthase, cyclin-G, pantothenate kinase-1 (PANK1), respectively. 3 proteins were up-regulated and 5 proteins were down-regulated from the T1DM rats. Of the 8 proteins identified, MLCK was confirmed by Western blot and IHC.

Conclusion: This work demonstrates that a comprehensive strategy of proteomic identification should be a useful tool for understanding of diabetic encephalopathy mechanism. And the differential proteins such as MLCK may be give clues about the pathogenesis of diabetic encephalopathy.

Key words

proteome - type 1 diabetes mellitus - brain - streptozotocin

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Correspondence

W. PanMD 

Department of Medical

Laboratory

Guiyang Medical College

550004 Guiyang

Phone: +86 0851 6780726

Fax: +86 0851 6761424

Email: pw_6602@sina.com