Freewheel Training Alters Mouse Hippocampal Cytokines

N. Pervaiz; L. Hoffman-Goetz

doi:10.1055/s-0031-1279780

International Journal of Sports Medicine, Table of Contents

Int J Sports Med 2011; 32(11): 889-895
DOI: 10.1055/s-0031-1279780

Immunology

Georg Thieme Verlag KG Stuttgart · New York

Freewheel Training Alters Mouse Hippocampal Cytokines

N. Pervaiz

¹University of Waterloo, Health Studies and Gerontology, Waterloo, Ontario, Canada

,

L. Hoffman-Goetz

¹University of Waterloo, Health Studies and Gerontology, Waterloo, Ontario, Canada

› Author Affiliations

Abstract

The effects of 16 weeks of voluntary wheel running in healthy female mice on hippocampal expression of pro-(TNF-α, IL-1β, IL-12) and anti-(IL-10, IL-1ra) inflammatory and pleiotropic (IL-6) cytokines and apoptotic status of specific cell subsets (CD45⁺, CD11b⁺) were studied. Mice were assigned to wheel running (WR; n=20) or a control condition (No WR; n=22) and sacrificed after the 16 weeks. Data collected included measures of training status (running volume, body weight, run-to-exhaustion time, and skeletal muscle cytochrome c oxidase activity), flow cytometric analysis of cell phenotypes and apoptosis (CD45⁺, CD11b⁺, Annexin⁺, Annexin⁺/PI⁺, PI⁺), and cytokine concentrations in cell lysates. WR mice had measurable training effects and significantly lower TNF-α (p<0.05) and higher IL-6 (p<0.05), IL-1ra (p<0.05) and IL-12 (p<0.05) expression in the hippocampus compared to controls. IL-1β, IL-10, and the percent of apoptotic and dead cells did not change due to training. Taken together, and in relation to the complex interactions between cytokines, the results suggest a possible mechanism whereby exercise training may buffer from dementia and cognitive decline through changes in the central cytokine milieu in the hippocampus.

Key words

voluntary exercise - hippocampus - microglia - TNF-α - IL-6 - IL-1ra

Full Text

References

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