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Neuropediatrics 2011; 42(06): 222-226
DOI: 10.1055/s-0031-1295480
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Total Antioxidant Capacity and Total Oxidant Status in Perinatal Asphyxia in Relation to Neurological Outcome

O. Aydemir
1   Department of Neonatology, Zekai Tahir Burak Maternity Teaching Hospital, Ankara, Turkey
,
M. Akar
1   Department of Neonatology, Zekai Tahir Burak Maternity Teaching Hospital, Ankara, Turkey
,
N. Uras
1   Department of Neonatology, Zekai Tahir Burak Maternity Teaching Hospital, Ankara, Turkey
,
Z. Eras
2   Department of Developmental Pediatrics, Zekai Tahir Burak Maternity Teaching Hospital, Ankara, Turkey
,
O. Erdeve
1   Department of Neonatology, Zekai Tahir Burak Maternity Teaching Hospital, Ankara, Turkey
,
S. S. Oguz
1   Department of Neonatology, Zekai Tahir Burak Maternity Teaching Hospital, Ankara, Turkey
,
U. Dilmen
1   Department of Neonatology, Zekai Tahir Burak Maternity Teaching Hospital, Ankara, Turkey
› Author Affiliations
Further Information

Publication History

received 10 July 2011

accepted 31 October 2011

Publication Date:
05 December 2011 (online)

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Abstract

This study aimed to investigate the global oxidant/antioxidant status of infants with perinatal asphyxia and its relation to neurological outcomes. A prospective controlled study including term infants with perinatal asphyxia was conducted. Blood samples were obtained from patients and controls at 6–24 h and on the 3rd day of life for TAC and TOS measurement and OSI values were calculated. Neurodevelopment was evaluated at 12 months of age in survivors using Bayley scales of infant development II (BSID II). 17 term infants with perinatal asphyxia and 17 healthy controls were enrolled. On the first day of life TAC, TOS and OSI were significantly higher in patients with perinatal asphyxia (p<0.001). Total antioxidant capacity decreased significantly on day 3 compared to first day of life in the patient group (p=0.04). Infants with seizures and abnormal amplitude-integrated electroencephalography recordings had higher TOS and OSI levels in the 1st day. There was no correlation between TAC, TOS and OSI levels and BSID II scores. In conclusion oxidant/antioxidant balance is disturbed in favour of oxidants in perinatal asphyxia. Degree of oxidative stress is related to severity of neurological involvement in the first days of life.

Key words

hypoxic-ischemic encephalopathy - newborn - neurological outcome - oxidative stress - seizures
 

  • References

  • 1 Al Naqueeb N, Edwards AD, Cowan F et al. Assessment of neonatal encephalopathy by amplitude integrated electroencephalography. Pediatrics 1999; 103: 1263-1271
    CrossrefPubMedSearch in Google Scholar
  • 2 Azzopardi DV, Strohm B, Edwards AD et al. TOBY Study Group. Moderate hypothermia to treat perinatal asphyxial encephalopathy. N Engl J Med 2009; 361: 1349-1358
    CrossrefPubMedSearch in Google Scholar
  • 3 Banupriya C, Ratnakar S, Doureradjou P et al Can urinary excretion rate of malondialdehyde, uric acid and protein predict the severity and impending death in perinatal asphyxia?. Clin Biochem 2008; 41: 968-973
    CrossrefPubMedSearch in Google Scholar
  • 4 Berger R, Garnier Y. Perinatal brain injury. J Perinat Med 2000; 28: 261-285
    CrossrefPubMedSearch in Google Scholar
  • 5 Calamandrei G, Venerosi AP, Valanzano A et al. Increased brain levels of F2-isoprostane are an early marker of behavioral sequels in a rat model of global perinatal asphyxia. Pediatr Res 2004; 55: 85-92
    CrossrefPubMedSearch in Google Scholar
  • 6 Capani F, Loidl CF, Aguirre F et al. Changes in reactive oxygen species (ROS) production in rat brain during global perinatal asphyxia: an ESR study. Brain Res 2001; 914: 204-207
    CrossrefPubMedSearch in Google Scholar
  • 7 Capani F, Loidl CF, Piehl LL et al. Long term production of reactive oxygen species during perinatal asphyxia in the rat central nervous system: effects of hypothermia. Int J Neurosci 2003; 113: 641-654
    CrossrefPubMedSearch in Google Scholar
  • 8 Castillo-Meléndez M, Chow JA, Walker DW. Lipid peroxidation, caspase-3 immunoreactivity, and pyknosis in late-gestation fetal sheep brain after umbilical cord occlusion. Pediatr Res 2004; 55: 864-871
    CrossrefPubMedSearch in Google Scholar
  • 9 Derks JB, Oudijk MA, Torrance HL et al. Allopurinol reduces oxidative stress in the ovine fetal cardiovascular system after repeated episodes of ischemia-reperfusion. Pediatr Res 2010; 68: 374-380
    PubMedSearch in Google Scholar
  • 10 Domoki F, Oláh O, Zimmermann A et al. Hydrogen is neuroprotective and preserves cerebrovascular reactivity in asphyxiated newborn pigs. Pediatr Res 2010; 68: 387-392
    PubMedSearch in Google Scholar
  • 11 Doroszko A, Polewicz D, Cadete VJ et al. Neonatal asphyxia induces the nitration of cardiac myosin light chain 2 that is associated with cardiac systolic dysfunction. Shock 2010; 34: 592-600
    CrossrefPubMedSearch in Google Scholar
  • 12 Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem 2004; 37: 277-285
    Search in Google Scholar
  • 13 Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005; 38: 1103-1111
    CrossrefPubMedSearch in Google Scholar
  • 14 Gathwala G, Marwah A, Gahlaut V et al. Effect of high-dose phenobarbital on oxidative stress in perinatal asphyxia: an open label randomized controlled trial. Indian Pediatr 2010; Nov 30. pii: S097475590900675-1. [Epub ahead of print]
    PubMedSearch in Google Scholar
  • 15 Harma M, Harma M, Erel O. Measurement of the total antioxidant response in preeclampsia with a novel automated method. Eur J Obstet Gynecol Reprod Biol 2005; 118: 47-51
    CrossrefPubMedSearch in Google Scholar
  • 16 Ikeda T, Choi BH, Yee S et al. Oxidative stress, brain white matter damage and intrauterine asphyxia in fetal lambs. Int J Dev Neurosci 1999; 17: 1-14
    CrossrefPubMedSearch in Google Scholar
  • 17 Jacobs S, Hunt R, Tarnow-Mordi W et al. Cooling for newborns with hypoxic ischaemic encephalopathy. Cochrane Database Syst Rev 2007; (04) CD003311
    PubMedSearch in Google Scholar
  • 18 Kumar A, Ramakrishna SV, Basu S et al. Oxidative stress in perinatal asphyxia. Pediatr Neurol 2008; 38: 181-185
    CrossrefPubMedSearch in Google Scholar
  • 19 Levene MI, De Vries LS. Hypoxic-ischemic rncephalopathy. In: Martin RJ, Fanaroff AA, Walsh MC. (Eds.) Neonatal-Perinatal Medicine. St. Louis, Missouri. Mosby: Elsevier; 2011: 952-975
    Search in Google Scholar
  • 20 Mondal N, Bhat BV, Koner B et al. Oxidative stress in perinatal asphyxia in relation to outcome. Indian J Pediatr 2010; 77: 515-517
    CrossrefPubMedSearch in Google Scholar
  • 21 Ogihara T, Hirano K, Ogihara H et al. Non-protein-bound transition metals and hydroxyl radical generation in cerebrospinal fluid of newborn infants with hypoxic ischemic encephalopathy. Pediatr Res 2003; 53: 594-599
    CrossrefPubMedSearch in Google Scholar
  • 22 Papazisis G, Pourzitaki C, Sardeli C et al. Deferoxamine decreases the excitatory amino acid levels and improves the histological outcome in the hippocampus of neonatal rats after hypoxia–ischemia. Pharmacol Res 2008; 57: 73-78
    CrossrefPubMedSearch in Google Scholar
  • 23 Perrone S, Szabó M, Bellieni CV et al. Whole body hypothermia and oxidative stress in babies with hypoxic-ischemic brain injury. Pediatr Neurol 2010; 43: 236-240
    CrossrefPubMedSearch in Google Scholar
  • 24 Piscopo P, Bernardo A, Calamandrei G et al. Altered expression of cyclooxygenase-2, presenilins and oxygen radical scavenging enzymes in a rat model of global perinatal asphyxia. Exp Neurol 2008; 209: 192-198
    CrossrefPubMedSearch in Google Scholar
  • 25 Sarnat HBO, Sarnat MS. Neonatal encephalopathy following fetal distress: a clinical and electroencephalographic study. Arch Neurol 1976; 33: 696-705
    CrossrefPubMedSearch in Google Scholar
  • 26 Taylor DL, Edwards AD, Mehmet H. Oxidative metabolism, apoptosis and perinatal brain injury. Brain Pathol 1999; 9: 93-117
    PubMedSearch in Google Scholar
  • 27 Torrance HL, Benders MJ, Derks JB et al. Maternal allopurinol during fetal hypoxia lowers cord blood levels of the brain injury marker S-100B. Pediatrics 2009; 124: 350-357
    CrossrefPubMedSearch in Google Scholar