Intervention of TLR4 Signal Pathway Cytokines in Severe Liver Injury with Obstructive Jaundice in Rats

Abstract

Obstructive jaundice is a common surgical disease and a variety of end-stage severe liver injuries still lack effective treatments. Compared to traditional liver transplantation therapy, herbal treatment is noninvasive and has fewer side effects. Research results have shown that a modified major decoction of bupleurum can reduce the toxic reaction of obstructive jaundice, even though the mechanism is unclear. A period of chronic exercise training can significantly reduce TLR4 expression in mononuclear cells and the secretion of inflammatory cell factors. Our study administered a modified major decoction of bupleurum in combination with exercise in rats with obstructive jaundice and the results indicated that applying a major bupleurum decoction in combination with moderately intense aerobic exercise showed a beneficial effect on adjusting the expression of liver inflammatory cytokines, which thus improved immunity and finally reduced the liver injury of rats with obstructive jaundice.

Key words

modified major decoction of bupleurum - medium-intensity aerobic exercise - obstructive jaundice - TLR4

• References

• 1 Arosio B, Gagliano N, Fusaro LM, Parmeggiani L, Tagliabue J, Galetti P, De Castri D, Moscheni C, Annoni G. Aloe-Emodin quinone pretreatment reduces acute liver injury induced by carbon tetrachloride. Pharmacol Toxicol 2000; 87: 229-233
• 2 Bedrord TG, Tipton CM, Wilson NC, Oppliger RA, Gisolfi CV. Maximum oxygen consumption of rats and its changes with various experimental procedures. J Appl Physiol 1979; 7: 1278-1283
• 3 Chungen Y. Sports and expression of toll-like receptor. Chin J Sports Med 2007; 26: 644
• 4 Ferry A, Rieu P, Laziri F, el Habazi A, Le Page C, Rieu M. Effect of moderat exercise on rat T-cells. Eur J Appl Physiol 1992; 65: 464-468
• 5 Gao Z, Huang K, Yang X, Xu H. Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of scutellaria baicalensis georgi. Bichim Biophys Acta 1999; 1472: 643-650
• 6 Haiyan B. Approach on Mechanism of Receding Hot and Damp Jaundice of Haoqinqingdan Decoction. Journal of Henan University of Chinese Medicine 2009; 24: 36
• 7 Harriss DJ, Atkinson G. Update – Ethical standards in sport and exercise science research. Int J Sports Med 2011; 32: 819-821
• 8 Kennedy JA, Clements WD, Kirk SJ, McCaigue MD, Campbell GR, Erwin PJ, Halliday MI, Rowlands BJ. Characterization of the Kupffer cell response to exogenous endotoxin in a rodent model of obstructive jaundice. Br J Surg 1999; 86: 628-633
• 9 Li M, Yating W. Critical role of toll-like receptors in regulation of immune response. J Appl Clin Pediatr 2009; 24: 1685-1688
• 10 Min L, Libo Y, Pingguo L, Wenxiu Z, Xiaoming W, Zhengyu Y, Xiang G. LPS increases the liver injury via TLR4-dependent manner in mice with acute obstructive jaundice. J Hep Surg 2010; 18: 226
• 11 Musheng L, Sanming W, Shiting B, Huilai M. Effects of L-arginine and modified major decoction of bupleurum on endotoxemia in the rat with obstructive jaundice. China J Exp Surg 2004; 10: 1202-1203
• 12 Ricken KH, Rieder T, Hauck G, Kindermann W. Changes in lymphocyte subpopulations after prolonged exercise. Int J Sports Med 1990; 11: 132-135
• 13 Roiand CR, Goss JA, Mangino MJ, Hafenrichter D, Flye MW. Autoregulation by eicosanoids of human Kupffer cell secretory products. A study of interleukin-1, interleukin-6, tumor necrosis factor-alpha, transforming growth factor-beta, and nitric oxide. Ann Surg 1994; 219: 389
• 14 Seki E, De Minicis S, Osterreicher CH, Kluwe J, Osawa Y, Brenner DA, Schwabe RF. TLR4 enhances TGF2β signaling and hepatic fibrosis. Nat Med 2007; 13: 1324-1332
• 15 Shouhua Z, Caixian L, Chunxing Z, Hong D, Hang Z, Lei L, Chen Y. Establishment of a mouse model of biliary obstruction and its dynamic observations. J S Med Univ 2008; 28: 1579-1581
• 16 Wang H, Li ZY, Wu HS, Wang Y, Jiang CF, Zheng QC, Zhang JX. Endogenous danger signals trigger hepatic ischemia/reperfusion injury through toll-like receptor 4/nuclear factor kappa B pathway. China Med J (Engl) 2007; 120: 509-514
• 17 Wang L, Xu JB, Wu HS, Zhang JX, Zhang JH, Tian Y, Wang CY. The relationship between activation of TLR4 and partial hepatic ischemia/reperfusion injury in mice. Hep Panc Dis Int 2006; 5: 101-104
• 18 Yanping Z. The research progress of the body’s immune function in obstructive jaundice. Jiangxi Medical Journal 2009; 11: 1149
• 19 Yi X, Yi T, Haixin Q. Effects of modified major decoction of bumleurum on toll-like receptor 4 of liver in rat with acute cholangitis of severe type(ACST). Jiangsu Medical Journal 2009; 35: 833
• 20 Yongqing P. Try to talk about modified major decoction of bupleurum for clearing heat prescription. Journal of Beijing Chinese Medicine University 1984; 7: 24-25
• 21 Zhao J, Lu W, Guo H, Ming S. Liver fibrosis and research progress of cytokines. China Medical Herald 2008; 5: 20