Chemical Constituents and Anticancer Effects of the Essential Oil from Leaves of Xylopia laevigata

Jullyana de S. S. Quintans; Bruno M. Soares; Rosana P. C. Ferraz; Allan C. A. Oliveira; Thanany B. da Silva; Leociley R. A. Menezes; Marília F. C. Sampaio; Ana Paula do N. Prata; Manoel O. Moraes; Claudia Pessoa; Angelo R. Antoniolli; Emmanoel V. Costa; Daniel P. Bezerra

doi:10.1055/s-0032-1328091

Planta Medica, Inhaltsverzeichnis

Planta Med 2013; 79(02): 123-130
DOI: 10.1055/s-0032-1328091

Biological and Pharmacological Activity

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Chemical Constituents and Anticancer Effects of the Essential Oil from Leaves of Xylopia laevigata

Jullyana de S. S. Quintans

¹Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil

,

Bruno M. Soares

²Department of Physiology and Pharmacology, Federal University of Ceará, School of Medicine, Fortaleza, Ceará, Brazil

,

Rosana P. C. Ferraz

¹Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil

,

Allan C. A. Oliveira

¹Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil

,

Thanany B. da Silva

³Department of Chemistry, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil

,

Leociley R. A. Menezes

³Department of Chemistry, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil

,

Marília F. C. Sampaio

³Department of Chemistry, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil

,

Ana Paula do N. Prata

⁴Department of Biology, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil

,

Manoel O. Moraes

²Department of Physiology and Pharmacology, Federal University of Ceará, School of Medicine, Fortaleza, Ceará, Brazil

,

Claudia Pessoa

²Department of Physiology and Pharmacology, Federal University of Ceará, School of Medicine, Fortaleza, Ceará, Brazil

,

Angelo R. Antoniolli

¹Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil

,

Emmanoel V. Costa

³Department of Chemistry, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil

,

Daniel P. Bezerra

¹Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil

› Institutsangaben

Abstract

Xylopia laevigata, popularly known as “meiú” and “pindaíba”, is a medicinal plant used in the folk medicine of the Brazilian Northeast for several purposes. The chemical constituents of the essential oil from leaves of X. laevigata, collected from wild plants growing at three different sites of the remaining Atlantic forest in Sergipe State (Brazilian Northeast), were analyzed by GC/FID and GC/MS. The effect of the essential oil samples was assessed on tumor cells in culture, as well on tumor growth in vivo. All samples of the essential oil were dominated by sesquiterpene constituents. A total of 44 compounds were identified and quantified. Although some small differences were observed in the chemical composition, the presence of γ-muurolene (0.60–17.99 %), δ-cadinene (1.15–13.45 %), germacrene B (3.22–7.31 %), α-copaene (3.33–5.98 %), germacrene D (9.09–60.44 %), bicyclogermacrene (7.00–14.63 %), and (E)-caryophyllene (5.43–7.98 %) were verified as major constituents in all samples of the essential oil. In the in vitro cytotoxic study, the essential oil displayed cytotoxicity to all tumor cell lines tested, with the different samples displaying a similar profile; however, they were not hemolytic or genotoxic. In the in vivo antitumor study, tumor growth inhibition rates were 37.3–42.5 %. The treatment with the essential oil did not significantly affect body weight, macroscopy of the organs, or blood leukocyte counts. In conclusion, the essential oil from the leaves of X. laevigata is chemically characterized by the presence of γ-muurolene, δ-cadinene, germacrene B, α-copaene, germacrene D, bicyclogermacrene, and (E)-caryophyllene as major constituents and possesses significant in vitro and in vivo anticancer potential.

Key words

Xylopia laevigata - Annonaceae - essential oil - chemical composition - antitumor

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