Photodynamic Effect of Hypericin after Topical Application in the
                        Ex Ovo Quail Chorioallantoic Membrane Model

Ivan Čavarga; Boris Bilčík; Pavel Výboh; Monika Záškvarová; Dušan Chorvát; Peter Kasák; Peter Mlkvý; Anton Mateašík; Alžbeta Chorvátová; Pavol Miškovský

doi:10.1055/s-0033-1360174

Planta Medica, Inhaltsverzeichnis

Planta Med 2014; 80(01): 56-62
DOI: 10.1055/s-0033-1360174

Biological and Pharmacological Activity

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Photodynamic Effect of Hypericin after Topical Application in the Ex Ovo Quail Chorioallantoic Membrane Model

Ivan Čavarga

¹Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovakia

²St Elizabeth Oncological Institute, Bratislava, Slovakia

,

Boris Bilčík

¹Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovakia

,

Pavel Výboh

¹Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovakia

,

Monika Záškvarová

¹Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovakia

,

Dušan Chorvát

³International Laser Centre, Bratislava, Slovakia

,

Peter Kasák

⁴Polymer Institute, Slovak Academy of Sciences, Bratislava, Slovakia

,

Peter Mlkvý

²St Elizabeth Oncological Institute, Bratislava, Slovakia

,

Anton Mateašík

³International Laser Centre, Bratislava, Slovakia

,

Alžbeta Chorvátová

³International Laser Centre, Bratislava, Slovakia

,

Pavol Miškovský

⁵Dept. of Biophysics, Faculty of Sciences, P. J. Šafárik University, Košice, Slovakia

⁶Center for Interdisciplinary Biosciences, P. J. Šafárik University, Košice, Slovakia

› Institutsangaben

Abstract

Photosensitizing properties of hypericin are well known, and the chicken chorioallantoic membrane has previously been used to test photodynamic effects of hypericin and other substances. In our study the photodynamic effect of hypericin in the ex ovo quail chorioallantoic membrane model was evaluated. Steady-state and time-resolved fluorescence spectroscopy of hypericin solution in PEG-400 and its mixture in PBS was performed to assess and characterize the process of aggregation and disaggregation of hypericin during the drug formulation preparation. A therapeutical formulation (2 µg/g of embryo weight) was topically applied on CAM into the silicone ring. Hypericin was excited by diode laser with wavelength 405 nm, fluence rate 140 mW/cm², and fluence 16.8 J/cm². Hypericin in 100 % PEG-400 exhibits typical fluorescence spectra with a maximum of about 600 nm, while hypericin 10% PEG-400 formulation exhibits almost no fluorescence. Time resolved spectra analysis showed fluorescence decay of hypericin in 100 % PEG-400 solution with a mean lifetime of 5.1 ns and in 10 % PEG 4.1 ns. Damage of quail chorioallantoic membrane vasculature after photodynamic therapy ranged from hemorrhage and vanishing of capillary vessels to thrombosis, lysis, and hemorrhage of larger vessels.

The presented findings suggest that quail embryos can be used as a suitable model to test the effect of hypericin and other photodynamic compounds.

Key words

hypericin - Japanese quail - chorioallantoic membrane - photodynamic therapy - aggregation - Hypericum - Hypericaceae

Volltext

Referenzen

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