Planta Med 2014; 80(04): 343-350
DOI: 10.1055/s-0033-1360337
Biological Screening
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Inhibition of Bacterial Quorum Sensing and Biofilm Formation by Extracts of Neotropical Rainforest Plants

Chieu Anh Ta
1   Laboratory for Analysis of Natural and Synthetic Environmental Toxins (LANSET), Department of Biology, University of Ottawa, Ottawa, Canada
,
Marie Freundorfer
1   Laboratory for Analysis of Natural and Synthetic Environmental Toxins (LANSET), Department of Biology, University of Ottawa, Ottawa, Canada
,
Thien-Fah Mah
2   Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada
,
Marco Otárola-Rojas
3   Herbario Juvenal Valerio Rodriguez, Universidad Nacional, Heredia, Costa Rica
,
Mario Garcia
3   Herbario Juvenal Valerio Rodriguez, Universidad Nacional, Heredia, Costa Rica
,
Pablo Sanchez-Vindas
3   Herbario Juvenal Valerio Rodriguez, Universidad Nacional, Heredia, Costa Rica
,
Luis Poveda
3   Herbario Juvenal Valerio Rodriguez, Universidad Nacional, Heredia, Costa Rica
,
J. Alan Maschek
4   Department of Chemistry, University of South Florida, Tampa, FL, United States
,
Bill J. Baker
4   Department of Chemistry, University of South Florida, Tampa, FL, United States
,
Allison L. Adonizio
5   Center for Ethnobiology and Natural Products (CENaP), Department of Biological Sciences, Florida International University, Miami, FL, United States
,
Kelsey Downum
5   Center for Ethnobiology and Natural Products (CENaP), Department of Biological Sciences, Florida International University, Miami, FL, United States
,
Tony Durst
6   Department of Chemistry, University of Ottawa, Ottawa, Canada
,
John T. Arnason
1   Laboratory for Analysis of Natural and Synthetic Environmental Toxins (LANSET), Department of Biology, University of Ottawa, Ottawa, Canada
› Author Affiliations
Further Information

Publication History

received 24 October 2013
revised 07 January 2014

accepted 09 January 2014

Publication Date:
31 January 2014 (online)

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Abstract

Bacterial biofilms are responsible for many persistent infections by many clinically relevant pathogens such as Staphylococcus aureus and Pseudomonas aeruginosa. Biofilms are much more resistant to conventional antibiotics than their planktonic counterparts. Quorum sensing, an intercellular communication system, controls pathogenesis and biofilm formation in most bacterial species. Quorum sensing provides an important pharmacological target since its inhibition does not provide a selective pressure for resistance. In this study, we investigated the quorum sensing and biofilm inhibitory activities of 126 plant extracts from 71 species collected from neotropical rainforests in Costa Rica. Quorum sensing and biofilm interference were assessed using a modified disc diffusion bioassay with Chromobacterium violaceum ATCC 12 472 and a spectrophotometric bioassay with Pseudomonas aeruginosa PA14, respectively. Species with significant anti-quorum sensing and/or anti-biofilm activities belonged to the Meliaceae, Melastomataceae, Lepidobotryaceae, Sapindaceae, and Simaroubaceae families. IC50 values ranged from 45 to 266 µg/mL. Extracts of these active species could lead to future development of botanical treatments for biofilm-associated infections.