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Planta Med 2014; 80(04): 330-336
DOI: 10.1055/s-0033-1360362
Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Comprehensive Chemical Analysis of the Rhizomes of Drynaria fortunei by Orthogonal Pre-Separation and Liquid Chromatography Mass Spectrometry

Xue Qiao
1   State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China
,
Xiong-hao Lin
1   State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China
,
Yong-hong Liang
1   State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China
,
Jing Dong
2   Shimadzu International Trading (Shanghai) Co. Ltd., Beijing Office, Beijing, China
,
De-an Guo
1   State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China
,
Min Ye
1   State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China
› Author Affiliations
Further Information

Publication History

received 08 September 2013
revised 28 November 2013

accepted 20 January 2014

Publication Date:
18 February 2014 (online)

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Abstract

The chemical composition of Drynaria fortunei, a traditional Chinese herbal medicine, is very complicated. In order to separate these chemicals to obtain their structural information, an orthogonal sample enrichment procedure was established. The ethyl acetate extract of D. fortunei was pre-separated by Sephadex LH-20 × polyamide columns to yield 15 fractions. These fractions were analyzed successively using a reversed-phase Agilent Zorbax SB-C18 column, coupled with diode array detection and electrospray ionization tandem mass spectrometry. The method reduced co-elution and enriched minor compounds on the basis of their chemical features. A total of 369 compounds were detected by LC/MSn, compared to less than 50 compounds without pre-separation. The pretreatment facilitated the analytical separation of flavonoids, proanthocyanidins, triterpenoids, phenolic acids, and lignans in D. fortunei, and allowed a comprehensive chemical profiling of these constituents. This method could be applied to other multicomponent herbal extracts.

Key words

Drynaria fortune - Polypodiaceae - pre-separation - proanthocyanidins - orthogonal chromatography - HPLC-DAD-ESI-MSn

Supporting Information

 

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