Circulating CD3+56+ Cell Subset in Pre-diabetes

M. Dworacka; A. Wesołowska; E. Wysocka; H. Winiarska; S. Iskakova; G. Dworacki

doi:10.1055/s-0033-1363233

Experimental and Clinical Endocrinology & Diabetes, Table of Contents

Exp Clin Endocrinol Diabetes 2014; 122(2): 65-70
DOI: 10.1055/s-0033-1363233

Article

Circulating CD3⁺56⁺ Cell Subset in Pre-diabetes

M. Dworacka

¹Department of Pharmacology Poznan University of Medical Sciences, Poznań, Poland

,

A. Wesołowska

¹Department of Pharmacology Poznan University of Medical Sciences, Poznań, Poland

,

E. Wysocka

²Department of Chemistry and Clinical Biochemistry Poznan University of Medical Sciences, Poznań, Poland

,

H. Winiarska

¹Department of Pharmacology Poznan University of Medical Sciences, Poznań, Poland

,

S. Iskakova

³Department of Pharmacology, West Kazakhstan State Medical University, Aktobe, Kazakhstan

,

G. Dworacki

⁴Department of Clinical Immunology Poznan University of Medical Sciences, Poznań, Poland

› Author Affiliations

Abstract

Background:

The CD3⁺56⁺ cells are a small but significant population of T lymphocytes encompassing NKT-like and NKT cells, which may play the essential role at the very early stages of atherosclerotic plaque development. The frequency and activity of CD3⁺56⁺ cells in atherosclerosis-inducing dysglycaemic disease (diabetes type 2 or pre-diabetes) is largely unknown.

Methods:

We analysed CD3⁺56⁺ cell count, granzyme, perforin and annexin V profiles in the peripheral blood from a group of patients with pre-diabetes, with diabetes type 2 and from non-dysglycaemic controls. Measurements were made of fasting glucose levels, HbA₁c, 1,5-anhydroglucitol and lipid profile.

Results:

The mean counts of CD3⁺56⁺ cells were significantly higher in patients with pre-diabetes compared to both patients with diabetes and to control group. There was an increase in the number of CD3⁺56⁺ cells producing granzyme and perforin in pre-diabetic patients compared to other groups, while there were no difference in annexin V⁺ populations within examined groups. It was confirmed that CD3⁺56⁺ cells count is modified by metabolic factors and their parameters, namely HbA₁c and 1,5-anhydroglucitol values.

Conclusion:

It could be stated that the alterations of CD3⁺ 56⁺ cells count in peripheral blood of pre-diabetic and type 2 diabetic patients are related to different grades of carbohydrate deteriorations – postprandial hyperglycaemia and chronic hyperglycaemia.

Key words

CD3⁺56⁺ cells - cardiovascular risk management - pre-diabetes - diabetes type 2 - 1,5-anhydroglucitol

Full Text

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