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Drug Res (Stuttg) 2014; 64(10): 563-568
DOI: 10.1055/s-0033-1363994
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

A Proteomic Analysis Using an Animal Model for Hyperlipidemia-related Erectile Dysfunction

Y.-G. Hwang
1   Ajou University, Yeongtong, Suwon, Gyunggi, Republic of Korea
,
H. Lee
1   Ajou University, Yeongtong, Suwon, Gyunggi, Republic of Korea
,
S. Lee
1   Ajou University, Yeongtong, Suwon, Gyunggi, Republic of Korea
,
H. K. Chung
2   Research Institutes of Dong-A ST Company, Gyunggi, Republic of Korea
,
K. K. Kang
2   Research Institutes of Dong-A ST Company, Gyunggi, Republic of Korea
,
S. H. Kim
2   Research Institutes of Dong-A ST Company, Gyunggi, Republic of Korea
› Author Affiliations
Further Information

Publication History

received 13 October 2013

accepted 20 December 2013

Publication Date:
22 January 2014 (online)

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Abstract

To investigate the pathogenesis of hyperlipidemia-related erectile dysfunction and the effects of DA-8159, a new phosphodiesterase-5 inhibitor, on protein expression, we performed a proteomic analysis of differentially expressed proteins in the corpus cavernosum of hyperlipidemic rats by two-dimensional electrophoresis-mass spectrometry. Rats were fed high-cholesterol diet and treated with 5 mg·kg−1·day−1 DA-8159 concurrently. After 5 months apparent hyperlipidemia and significantly decreased maximal intra-cavernous pressure were observed in the control group with the alteration of 8 proteins, which were restored by DA-8159 treatment. The proteins whose levels decreased >2-fold and attenuated by DA-8 159 were determined alcohol dehydrogenase, aldolase A, annexin 1, and tropomyosin-rat, whereas proteins increased>2-fold and recovered by DA-8159 were found to be aldehyde dehydrogenase complex, guanine deaminase, creatine kinase-B, and phosphoglycerate mutase type B subunit.

Key words

cholesterol diet - two-dimensional electrophoresis - mass spectrometry - phosphodiesterase-5 inhibitor
 

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