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Planta Med 2014; 80(07): 544-549
DOI: 10.1055/s-0034-1368445
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Aurantio-Obtusin Stimulates Chemotactic Migration and Differentiation of MC3T3-E1 Osteoblast Cells

Chethala N. Vishnuprasad
1   Department of Nano, Medical and Polymer Materials, College of Engineering, Yeungnam University, Gyeongsan, Republic of Korea
,
Tomoko Tsuchiya
2   YU-ECI Research Center for Medical Sciences, Yeungnam University, Gyeongsan, Republic of Korea
3   Dept. of Biochemistry and Molecular Biology, College of Medicine, Yeungnam University, Daegu, Republic of Korea
,
Shiro Kanegasaki
1   Department of Nano, Medical and Polymer Materials, College of Engineering, Yeungnam University, Gyeongsan, Republic of Korea
2   YU-ECI Research Center for Medical Sciences, Yeungnam University, Gyeongsan, Republic of Korea
,
Joon Ho Kim
1   Department of Nano, Medical and Polymer Materials, College of Engineering, Yeungnam University, Gyeongsan, Republic of Korea
,
Sung Soo Han
1   Department of Nano, Medical and Polymer Materials, College of Engineering, Yeungnam University, Gyeongsan, Republic of Korea
2   YU-ECI Research Center for Medical Sciences, Yeungnam University, Gyeongsan, Republic of Korea
› Author Affiliations
Further Information

Publication History

received 03 December 2013
revised 31 March 2014

accepted 01 April 2014

Publication Date:
19 May 2014 (online)

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Abstract

Osteoporosis is one of the major metabolic bone diseases and is among the most challenging noncommunicable diseases to treat. Although there is an increasing interest in identifying bioactive molecules for the prevention and management of osteoporosis, such studies principally focus only on differentiation and mineralization of osteoblasts or inhibition of osteoclast activity. Stimulation of osteoblast migration must be a promising osteoanabolic strategy for improved metabolic bone disease therapy. In this study, we show that an anthraquinone derivative, aurantio-obtusin, stimulated chemotactic migration of MC3T3-E1 osteoblast cells in a concentration-dependent manner. The use of a real-time chemotaxis analyzing system, TAXIScan, facilitated the evaluation of both velocity and directionality of osteoblast migration in response to the compound. Besides migration, the compound stimulated osteoblast differentiation and mineralization. Taken together, the data presented in this paper demonstrate that aurantio-obtusin is a promising osteoanabolic compound of natural origin with potential therapeutic applications in the prevention of osteoporosis and other metabolic bone diseases.

Key words

aurantio-obtusin - TAXIScan - chemotaxis - osteoblast migration - differentiation - mineralization
 

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