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Planta Med 2015; 81(09): 765-770
DOI: 10.1055/s-0035-1545913
Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

A Sensitive and Specific Indirect Competitive Enzyme-Linked Immunosorbent Assay for Detection of Paeoniflorin and Its Application in Pharmacokinetic Interactions between Paeoniflorin and Glycyrrhizinic Acid

Yan Zhao
1   School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, China
,
Huihua Qu
2   Scientific Research Experiment Center, Beijing University of Chinese Medicine, Beijing, China
,
Xueqian Wang
1   School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, China
,
Yue Zhang
1   School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, China
,
Wenchao Shan
3   College of Traditional Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, China
,
Yan Zhao *
1   School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, China
,
Qingguo Wang
1   School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, China
› Author Affiliations
Further Information

Publication History

received 29 October 2014
revised 06 January 2015

accepted 08 March 2015

Publication Date:
09 April 2015 (online)

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Erratum for: A Sensitive and Specific Indirect Competitive Enzyme-Linked Immunosorbent Assay for Detection of Paeoniflorin and Its Application in Pharmacokinetic Interactions between Paeoniflorin and Glycyrrhizinic AcidPlanta Med 2015; 81(09): E2-E2
DOI: 10.1055/s-0035-1546186

Abstract

This study aimed to develop an indirect competitive enzyme-linked immunosorbent assay based on monoclonal antibodies against paeoniflorin to study the effects of different doses of glycyrrhizinic acid on the pharmacokinetics of paeoniflorin in mice. An anti-paeoniflorin monoclonal antibody was produced from a hybridoma created through a fusion of splenocytes immunized with paeoniflorin-bovine serum albumin and conjugated with the hypoxanthine-aminopterin-thymidine-sensitive mouse myeloma cell line SP2/0. The resultant antibody was used to develop and validate a rapid, specific and sensitive, indirect competitive enzyme-linked immunosorbent assay for the measurement of paeoniflorin (linear range 4.8–312.5 ng · mL−1). The intraday and interday precision values of the indirect competitive ELISA method were well within the recommended range (≤ 10 %), and the recovery rate was, on average, 101.13 %. Pharmacokinetic parameters obtained from mouse blood samples at various intervals following the oral administration of paeoniflorin and glycyrrhizic acid at three doses (1 : 0.3, 1 : 1, 1 : 3, respectively) demonstrated that the highest dose of glycyrrhizic acid inhibits the absorption of paeoniflorin.

Key words

paeoniflorin - monoclonal antibody - enzyme-linked immunosorbent assay (ELISA) - pharmacokinetics - glycyrrhizic acid
 

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