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DOI: 10.1055/s-0035-1547219
Different Resistance-Training Regimens Evoked a Similar Increase in Myostatin Inhibitors Expression
Publication History
accepted after revision 29 January 2015
Publication Date:
30 March 2015 (online)
![](https://www.thieme-connect.de/media/sportsmed/201509/lookinside/thumbnails/4412_10-1055-s-0035-1547219-1.jpg)
Abstract
The aim of the present study was to investigate the effect of different resistance-training regimens (S or P) on the expression of genes related to the MSTN signaling pathway in physically-active men. 29 male subjects with at least 2 years of experience in strength training were assigned to either a strength-training group (S; n=11) or a power-training group (P; n=11). The control group (C; n=7) was composed of healthy physically-active males. The S and the P groups performed high- and low-intensity squats, respectively, 3 times per week, for 8 weeks. Muscle biopsies from the vastus lateralis muscle were collected before and after the training period. No change was observed in MSTN, ACTIIB, GASP-1 and FOXO-3 A gene expression after the training period. A similar increase in the gene expression of the inhibitory proteins of the MSTN signaling pathway, FLST (S: 4.2 fold induction and P: 3.7 fold induction, p<0.01) and FL-3 (S: 5.6 fold induction and P: 5.6 fold induction, p<0.01), was detected after the training period. SMAD-7 gene expression was similarly augmented after both training protocols (S: 2.5 fold induction; P: 2.8 fold induction; p<0.05). In conclusion, the resistance-training regimens (S and P) activated the expression of inhibitors of the MSTN signaling pathway in a similar manner.
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References
- 1 Allen DL, Unterman TG. Regulation of myostatin expression and myoblast differentiation by FoxO and SMAD transcription factors. Am J Physiol 2007; 292: C188-C199
- 2 Aoki MS, Soares AG, Miyabara EH, Baptista IL, Moriscot AS. Expression of genes related to myostatin signaling during rat skeletal muscle longitudinal growth. Muscle Nerve 2009; 40: 992-999
- 3 Amthor H, Nicholas G, McKinnell I, Kemp CF, Sharma M, Kambadur R, Patel K. Follistatin complexes Myostatin and antagonises Myostatin-mediated inhibition of myogenesis. Dev Biol 2004; 270: 19-30
- 4 Braun T, Gautel M. Transcriptional mechanisms regulating skeletal muscle differentiation, growth and homeostasis. Nat Rev Mol Cell Biol 2011; 12: 349-361
- 5 Burd NA, West DW, Staples AW, Atherton PJ, Baker JM, Moore DR, Holwerda AM, Parise G, Rennie MJ, Baker SK, Phillips SM. Low-load high volume resistance exercise stimulates muscle protein synthesis more than high-load low volume resistance exercise in young men. PLoS One 2010; 5: 12033
- 6 Bustin SA. Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): Trends and problems. J Mol Endocrinol 2002; 29: 23-39
- 7 Chapman DW, Newton M, McGuigan M, Nosaka K. Effect of lengthening contraction velocity on muscle damage of the elbow flexors. Med Sci Sports Exerc 2008; 40: 926-933
- 8 Chen Y, Lebrun JJ, Vale W. Regulation of transforming growth factor beta- and activin-induced transcription by mammalian Mad proteins. Proc Natl Acad Sci USA 1996; 93: 12992-12997
- 9 Dalbo VJ, Roberts MD, Hassell S, Kerksick CM. Effects of pre-exercise feeding on serum hormone concentrations and biomarkers of myostatin and ubiquitin proteasome pathway activity. Eur J Nutr 2013; 52: 477-487
- 10 Dalbo VJ, Roberts MD, Sunderland KL, Poole CN, Stout JR, Beck TW, Bemben M, Kerksick CM. Acute loading and aging effects on myostatin pathway biomarkers in human skeletal muscle after three sequential bouts of resistance exercise. J Gerontol A Biol Sci Med Sci 2011; 66: 855-865
- 11 Dreyer HC, Fujita S, Cadenas JG, Chinkes DL, Volpi E, Rasmussen BB. Resistance exercise increases AMPK activity and reduces 4E-BP1 phosphorylation and protein synthesis in human skeletal muscle. J Physiol 2006; 576: 613-624
- 12 Egan B, Zierath JR. Exercise metabolism and the molecular regulation of skeletal muscle adaptation. Cell Metab 2013; 17: 162-184
- 13 Farthing JP, Chilibeck PD. The effects of eccentric and concentric training at different velocities on muscle hypertrophy. Eur J Appl Physiol 2003; 89: 578-586
- 14 Forbes D, Jackman M, Bishop A, Thomas M, Kambadur R, Sharma M. Myostatin auto-regulates its expression by feedback loop through Smad7 dependent mechanism. J Cell Physiol 2006; 206: 264-272
- 15 Fry AC. The role of resistance exercise intensity on muscle fibre adaptations. Sports Med 2004; 34: 663-679
- 16 Gharakhanlou R, Saremi A, Omidfar K, Sharghi S, Gheraati Mr. Effect of resistance training on serum myostatin, GASP-1, IGF-I and IGFBP-3 in young men. J Movem Sci Spring-Summer 2009; 7: 67-79
- 17 Hansen J, Brandt C, Nielsen AR, Hojman P, Whitham M, Febbraio MA, Pedersen BK, Plomgaard P. Exercise induces a marked increase in plasma follistatin: evidence that follistatin is a contraction-induced hepatokine. Endocrinology 2011; 152: 164-171
- 18 Harriss DJ, Atkinson G. Ethical standards in sports and exercise science research: 2014 update. Int J Sports Med 2013; 34: 1025-1028
- 19 Hayashi H, Abdollah S, Qiu Y, Cai J, Xu YY, Grinnell BW, Richardson MA, Topper JN, Gimbrone Jr MA, Wrana JL, Falb D. The MAD-related protein Smad7 associates with the TGF-β receptor and functions as an antagonist of TGF-β signaling. Cell 1997; 89: 1165-1173
- 20 Hayette S, Gadoux M, Martel S, Bertrand S, Tigaud I, Magaud JP, Rimokh R. FLRG (follistatin-related gene), a new target of chromosomal rearrangement in malignant blood disorders. Oncogene 1998; 16: 2949-2954
- 21 Hill JJ, Qiu Y, Hewick RM, Wolfman NM. Regulation of myostatin in vivo by growth and differentiation factor-associated serum protein-1: a novel protein with protease inhibitor and follistatin domains. Mol Endocrinol 2003; 17: 1144-1154
- 22 Hulmi JJ, Ahtiainen JP, Kaasalainen T, Pollanen E, Hakkinen K, Alen M, Selanne H, Kovanen V, Mero AA. Postexercise myostatin and activin IIb mRNA levels: effects of strength training. Med Sci Sports Exerc 2007; 39: 289-297
- 23 Jensky NE, Sims JK, Dieli-Conwright CM, Sattler FR, Rice JC, Schroeder ET. Exercise does not influence myostatin and follistatin messenger RNA expression in young women. J Strength Cond Res 2010; 24: 522-530
- 24 Jensky NE, Sims JK, Rice JC, Dreyer HC, Schroeder ET. The influence of eccentric exercise on mRNA expression of skeletal muscle regulators. Eur J Appl Physiol 2007; 101: 473-480
- 25 Jespersen JG, Nedergaard A, Andersen LL, Schjerling P, Andersen JL. Myostatin expression during human muscle hypertrophy and subsequent atrophy: increased myostatin with detraining. Scand J Med Sci Sports 2011; 21: 215-223
- 26 Kyröläinen H, Avela J, McBride JM, Koskinen S, Andersen JL, Sipilä S, Takala TE, Komi PV. Effects of power training on muscle structure and neuromuscular performance. Scand J Med Sci Sports 2005; 15: 58-64
- 27 Kollias HD, McDermott JC. Transforming growth factor-beta and myostatin signaling in skeletal muscle. J Appl Physiol 2008; 104: 579-587
- 28 Lamas L, Aoki MS, Ugrinowitsch C, Campos GE, Regazzini M, Moriscot AS, Tricoli V. Expression of genes related to muscle plasticity after strength and power training regimens. Scand J Med Sci Sports 2010; 20: 216-225
- 29 Laurentino GC, Ugrinowitsch C, Roschel H, Aoki MS, Soares AG, Neves Jr M, Aihara AY, Fernandes Ada R, Tricoli V. Strength training with blood flow restriction diminishes myostatin gene expression. Med Sci Sports Exerc 2012; 44: 406-412
- 30 Leal ML, Lamas L, Aoki MS, Ugrinowitsch C, Ramos MS, Tricoli V, Moriscot AS. Effect of different resistance-training regimens on the WNT-signaling pathway. Eur J Appl Physiol 2011; 111: 2535-2545
- 31 Lee SJ, McPherron AC. Regulation of myostatin activity and muscle growth. Proc Natl Acad Sci USA 2001; 98: 9306-9311
- 32 Leger B, Cartoni R, Praz M, Lamon S, Deriaz O, Crettenand A, Gobelet C, Rohmer P, Konzelmann M, Luthi F, Russell AP. Akt signalling through GSK-3beta, mTOR and Foxo1 is involved in human skeletal muscle hypertrophy and atrophy. J Physiol 2006; 576: 923-933
- 33 Louis E, Raue U, Yang Y, Jemiolo B, Trappe S. Time course of proteolytic, cytokine, and myostatin gene expression after acute exercise in human skeletal muscle. J Appl Physiol 2007; 103: 1744-1751
- 34 Ma K, Mallidis C, Bhasin S, Mahabadi V, Artaza J, Gonzalez-Cadavid N, Arias J, Salehian B. Glucocorticoid-induced skeletal muscle atrophy is associated with upregulation of myostatin gene expression. Am J Physiol Metab 2003; 285: E363-E371
- 35 McPherron AC, Lee SJ. Double muscling in cattle due to mutations in the myostatin gene. Proc Natl Acad Sci USA 1997; 94: 12457-12461
- 36 McPherron AC, Lawler AM, Lee SJ. Regulation of skeletal muscle mass in mice by a new TGF-β superfamily member. Nature 1997; 387: 83-90
- 37 Murton AJ, Greenhaff PL. Resistance exercise and the mechanisms of muscle mass regulation in humans: acute effects on muscle protein turnover and the gaps in our understanding of chronic resistance exercise training adaptation. Int J Biochem Cell Biol 2013; 45: 2209-2214
- 38 O’Neil TK, Duffy LR, Frey JW, Hornberger TA. The role of phosphoinositide 3-kinase and phosphatidic acid in the regulation of mammalian target of rapamycin following eccentric contractions. J Physiol 2009; 587: 3691-3701
- 39 Reed SA, Sandesara PB, Senf SM, Judge AR. Inhibition of FoxO transcriptional activity prevents muscle fiber atrophy during cachexia and induces hypertrophy. FASEB J 2012; 26: 987-1000
- 40 Roth SM, Martel GF, Ferrell RE, Metter EJ, Hurley BF, Rogers MA. Myostatin gene expression is reduced in humans with heavy-resistance strength training: a brief communication. Exp Biol Med 2003; 228: 706-709
- 41 Santos AR, Neves Jr MT, Gualano B, Laurentino GC, Lancha Jr AH, Ugrinowitsch C, Lima FR, Aoki MS. Blood flow restricted resistance training attenuates myostatin gene expression in a patient with inclusion body myositis. Biol Sport 2014; 31: 121-124
- 42 Saremi A, Gharakhanloo R, Sharghi S, Gharaati MR, Larijani B, Omidfar K. Effects of oral creatine and resistance training on serum myostatin and GASP-1. Mol Cell Endocrinol 2010; 317: 25-30
- 43 Shepstone TN, Tang JE, Dallaire S, Schuenke MD, Staron RS, Phillips SM. Short-term high- vs. Low-velocity isokinetic lengthening training results in greater hypertrophy of the elbow flexors in young men. J Appl Physiol 2005; 98: 1768-1776
- 44 Schiffer T, Geisler S, Sperlich B, Struder HK. MSTN mRNA after varying exercise modalities in humans. Int J Sports Med 2011; 32: 683-687
- 45 Sorensen B, Jones JF, Vernon SD, Rajeevan MS. Transcriptional control of complement activation in an exercise model of chronic fatigue syndrome. Mol Med 2009; 15: 34-42
- 46 Tsuchida K, Arai KY, Kuramoto Y, Yamakawa N, Hasegawa Y, Sugino H. Identification and characterization of a novel follistatin-like protein as a binding protein for the TGF-beta family. J Biol Chem 2000; 275: 40788-40796
- 47 Williamson DL, Raue U, Slivka DR, Trappe S. Resistance exercise, skeletal muscle FOXO3 A, and 85-year-old women. J Gerontol A Biol Sci Med Sci 2010; 65: 335-343
- 48 Willoughby DS. Effects of heavy resistance training on myostatin mRNA and protein expression. Med Sci Sports Exerc 2004; 36: 574-582