Metabolic Profiles during an Oral Glucose Tolerance Test in Pregnant Women with and without Gestational Diabetes

R. Lehmann; T. Friedrich; G. Krebiehl; D. Sonntag; H.-U. Häring; A. Fritsche; A. M. Hennige

doi:10.1055/s-0035-1549887

Experimental and Clinical Endocrinology & Diabetes, Inhaltsverzeichnis

Exp Clin Endocrinol Diabetes 2015; 123(07): 483-438
DOI: 10.1055/s-0035-1549887

Article

Metabolic Profiles during an Oral Glucose Tolerance Test in Pregnant Women with and without Gestational Diabetes

R. Lehmann

¹Division of Clinical Chemistry and Pathobiochemistry, University Hospital Tuebingen, Tuebingen, Germany

²Department of Molecular Diabetology, Institute for Diabetes Research and Metabolic Diseases of the Helmholtz Zentrum München at the University of Tuebingen, Tuebingen, Germany

³German Center for Diabetes Research (DZD), Tuebingen, Germany

,

T. Friedrich

⁴Biocrates Life Sciences, Innsbruck, Austria

⁷New Affiliation: Institute for Experimental Genetics, Genome Analysis Center, German Research Center for Environmental Health of the Helmholtz Zentrum München, Neuherberg, Germany

,

G. Krebiehl

⁴Biocrates Life Sciences, Innsbruck, Austria

,

D. Sonntag

⁴Biocrates Life Sciences, Innsbruck, Austria

,

H.-U. Häring

³German Center for Diabetes Research (DZD), Tuebingen, Germany

⁵Department for Prevention and Therapy of Type 2 Diabetes, Institute for Diabetes Research and Metabolic Diseases of the Helmholtz Zentrum München at the University of Tuebingen, Tuebingen, Germany

⁶Department of Internal Medicine 4, Division of Endocrinology, Diabetology, Angiology and Nephrology, University Hospital Tuebingen, Germany

,

A. Fritsche

³German Center for Diabetes Research (DZD), Tuebingen, Germany

⁵Department for Prevention and Therapy of Type 2 Diabetes, Institute for Diabetes Research and Metabolic Diseases of the Helmholtz Zentrum München at the University of Tuebingen, Tuebingen, Germany

⁶Department of Internal Medicine 4, Division of Endocrinology, Diabetology, Angiology and Nephrology, University Hospital Tuebingen, Germany

,

A. M. Hennige

³German Center for Diabetes Research (DZD), Tuebingen, Germany

⁶Department of Internal Medicine 4, Division of Endocrinology, Diabetology, Angiology and Nephrology, University Hospital Tuebingen, Germany

› Institutsangaben

Abstract

Background/Aim: Gestational diabetes (GDM) is a complex metabolic condition associated with hyperpglycemia that is diagnosed in an oral glucose tolerance test (OGTT) during pregnancy. For a deeper understanding of the pathology of the disease, further investigations during pregnancy are required, ideally under metabolic challenging conditions.

Methods: We performed targeted metabolomics in a group of 24 well-matched women during an oral glucose tolerance test (OGTT). 231 plasma metabolites were profiled and compared to conventional clinical diagnostics.

Results: A pattern of 8 metabolites differed between GDM and healthy controls as early as 30 min in an OGTT (AUC 0.977±0.008), and an increase in acylcarnitine C18:0, decreased concentrations of diacyl phosphatidylcholines (PC aa) C34:4, PC aa C36:4, PC aa C38:5, Lyso PC C20:4 and arachidonic acid were associated with insulin resistance.

Conclusion: Our data suggest an additional value of metabolite pattern in the diagnosis of GDM and describe altered pathways that might be subjected to a more precise diagnosis and individualized therapy.

Key words

GDM - glucose tolerance test - pregnancy - targeted metabolomics - metabolites - insulin resistance

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