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Exp Clin Endocrinol Diabetes 2015; 123(07): 483-438
DOI: 10.1055/s-0035-1549887
Article
© Georg Thieme Verlag KG Stuttgart · New York

Metabolic Profiles during an Oral Glucose Tolerance Test in Pregnant Women with and without Gestational Diabetes

R. Lehmann
1   Division of Clinical Chemistry and Pathobiochemistry, University Hospital Tuebingen, Tuebingen, Germany
2   Department of Molecular Diabetology, Institute for Diabetes Research and Metabolic Diseases of the Helmholtz Zentrum München at the University of Tuebingen, Tuebingen, Germany
3   German Center for Diabetes Research (DZD), Tuebingen, Germany
,
T. Friedrich
4   Biocrates Life Sciences, Innsbruck, Austria
7   New Affiliation: Institute for Experimental Genetics, Genome Analysis Center, German Research Center for Environmental Health of the Helmholtz Zentrum München, Neuherberg, Germany
,
G. Krebiehl
4   Biocrates Life Sciences, Innsbruck, Austria
,
D. Sonntag
4   Biocrates Life Sciences, Innsbruck, Austria
,
H.-U. Häring
3   German Center for Diabetes Research (DZD), Tuebingen, Germany
5   Department for Prevention and Therapy of Type 2 Diabetes, Institute for Diabetes Research and Metabolic Diseases of the Helmholtz Zentrum München at the University of Tuebingen, Tuebingen, Germany
6   Department of Internal Medicine 4, Division of Endocrinology, Diabetology, Angiology and Nephrology, University Hospital Tuebingen, Germany
,
A. Fritsche
3   German Center for Diabetes Research (DZD), Tuebingen, Germany
5   Department for Prevention and Therapy of Type 2 Diabetes, Institute for Diabetes Research and Metabolic Diseases of the Helmholtz Zentrum München at the University of Tuebingen, Tuebingen, Germany
6   Department of Internal Medicine 4, Division of Endocrinology, Diabetology, Angiology and Nephrology, University Hospital Tuebingen, Germany
,
A. M. Hennige
3   German Center for Diabetes Research (DZD), Tuebingen, Germany
6   Department of Internal Medicine 4, Division of Endocrinology, Diabetology, Angiology and Nephrology, University Hospital Tuebingen, Germany
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Publikationsdatum:
14. Juli 2015 (online)

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Abstract

Background/Aim: Gestational diabetes (GDM) is a complex metabolic condition associated with hyperpglycemia that is diagnosed in an oral glucose tolerance test (OGTT) during pregnancy. For a deeper understanding of the pathology of the disease, further investigations during pregnancy are required, ideally under metabolic challenging conditions.

Methods: We performed targeted metabolomics in a group of 24 well-matched women during an oral glucose tolerance test (OGTT). 231 plasma metabolites were profiled and compared to conventional clinical diagnostics.

Results: A pattern of 8 metabolites differed between GDM and healthy controls as early as 30 min in an OGTT (AUC 0.977±0.008), and an increase in acylcarnitine C18:0, decreased concentrations of diacyl phosphatidylcholines (PC aa) C34:4, PC aa C36:4, PC aa C38:5, Lyso PC C20:4 and arachidonic acid were associated with insulin resistance.

Conclusion: Our data suggest an additional value of metabolite pattern in the diagnosis of GDM and describe altered pathways that might be subjected to a more precise diagnosis and individualized therapy.

Key words

GDM - glucose tolerance test - pregnancy - targeted metabolomics - metabolites - insulin resistance
 

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