Fenugreek Seed Extract Prevents Fat Deposition in Monosodium Glutamate (MSG)-Obese Rats

Abstract

Aim: To evaluate the preventive effects of an aqueous extract of Trigonella foenum-graecum seeds (AqE-TFG) on fat deposition and dyslipidemia in monosodium glutamate (MSG)-obese rats.

Methods: Neonatal male Wistar rats were injected with MSG (4 g/kg b. w., subcutaneously) from day 2 to 14 after birth, on alternate days. From day 43 to 70, MSG treated rats were administered orally with AqE-TFG (0.5 and 1.0 g/kg b. w.). The anthropometric and biochemical parameters were analyzed on day 71.

Results: Treatment with AqE-TFG produced significant (P < 0.05 or P < 0.01) reduction in body weight gain, Lee’s index, white adipose tissue (WAT) weights, adiposity index, blood glucose, serum insulin, leptin, lipids (low density lipoprotein cholesterol and very low density lipoprotein cholesterol), cardiac risk indexes (atherogenic index and coronary risk index), and homeostatic model assessment index. AqE-TFG treatment restored the activities of liver and epididymal WAT lipogenic enzymes (fatty acid synthetase and glucose-6-phosphate dehydrogenase) towards normal levels. Histological studies of liver also supported the experimental findings.

Conclusion: These findings demonstrated the preventive effect of AqE-TFG on fat deposition and dyslipidemia possibly by improvement in glucose and lipid metabolism, enhancement of insulin sensitivity and down regulation of lipogenic enzymes.

Key words

adiposity index - Lee’s index - lipogenesis - monosodium glutamate - neonatal - Trigonella foenum-graecum

• References

• 1 Kravchun P, Kadykova O, Gabisoniia T. The role of adipokines in formation of lipid and carbohydrate metabolic disorders in patients with cardiovascular disease. Georgian Med News 2012; 213: 26-31
• 2 Bechmann LP, Hannivoort RA, Gerken G et al. The interaction of hepatic lipid and glucose metabolism in liver diseases. J Hepatol 2012; 56: 952-964
• 3 Srivastava RA, Pinkosky SL, Filippov S et al. AMP-activated protein kinase: an emerging drug target to regulate imbalances in lipid and carbohydrate metabolism to treat cardio-metabolic diseases. J Lipid Res 2012; 53: 2490-2514
• 4 World Health Organization (WHO) . 2012 Obesity and overweight. http://www.who.int/mediacentre/factsheets/fs311/en/index.html
• 5 Kang JG, Park CY. Anti-obesity drugs: a review about their effects and safety. Diabetes Metab J 2012; 36: 13-25
• 6 Colon-Gonzalez F, Kim GW, Lin JE et al. Obesity pharmacotherapy: What is next?. Mol Aspects Med 2013; 34: 71-83
• 7 González-Castejón M, Rodriguez-Casado A. Dietary phytochemicals and their potential effects on obesity: A review. Pharmacol Res 2011; 64: 438-455
• 8 Birari RB, Bhutani KK. Pancreatic lipase inhibitors from natural source: unexplored potential. Drug Discov Today 2007; 12: 879-889
• 9 Warrier PK. Ocimum basilicum Linn. In Warrier PK, Nambiar VPK, Ramankutty C. (ed.). Indian Medicinal Plants. Madras: Orient Longman Ltd.; 1995. 1-5. 381-383
• 10 Bhandari U, Sharma JN, Grover JK. Effect of ethanolic extract of fenugreek seeds on hyperglycaemic rats. Asia Pacific J Pharmacol 1997; 12: 117-119
• 11 Kumar P, Bhandari U. Protective effect of Trigonella foenum-graecum Linn. on monosodium glutamate-induced dyslipidemia and oxidative stress in rats. Indian J Pharmacol 2013; 45: 136-140
• 12 Nardelli TR, Ribeiro RA, Balbo SL et al. Taurine prevents fat deposition and ameliorates plasma lipid profile in monosodium glutamate-obese rats. Amino Acids 2011; 41: 901-908
• 13 Matysková R, Maletínská L, Maixnerová J et al. Comparison of the obesity phenotypes related to monosodium glutamate effect on arcuate nucleus and/or the high fat diet feeding in C57BL/6 and NMRI mice. Physiol Res 2008; 57: 727-734
• 14 Onyema OO, Farombi EO, Emerole GO et al. Effect of vitamin E on monosodium glutamate induced hepatotoxicity and oxidative stress in rats. Indian J Biochem Biophys 2006; 43: 20-24
• 15 Bunyan J, Murrell EA, Shah PP. The induction of obesity in rodents by means of monosodium glutamate. Br J Nutr 1976; 35: 25-39
• 16 Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-685
• 17 Kumar P, Bhandari U, Jamadagni S. Fenugreek seed extract inhibit fat accumulation and ameliorates dyslipidemia in high fat diet-induced obese rats. Biomed Res Int 2014; 2014: 606021
• 18 Hamden K, Jaouadi B, Carreau S et al. Inhibitory effect of fenugreek galactomannan on digestive enzymes related to diabetes, hyperlipidemia, and liver-kidney dysfunctions. Biotechnol Bioprocess Eng 2010; 15: 407-413
• 19 Singleton VL, Rossi Jr JA. Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am J Enol Vitic 1965; 16: 144-158
• 20 Woisky RG, Salatino A. Analysis of propolis: some parameters and procedures for chemical quality control. J Apicult Res 1998; 37: 99-105
• 21 Richard WC, Paul FA, Celia DS. Monosodium glutamate neurotoxicity: A sex-specific impairment of blood pressure but not vasopressin in developing rats. Brain Res Bull 1986; 17: 51-58
• 22 Middha SK, Bhattacharjee B, Saini D et al. Protective role of Trigonella foenum graecum extract against oxidative stress in hyperglycemic rats. Eur Rev Med Pharmacol Sci 2011; 15: 427-435
• 23 Bernardis LL, Patterson BD. Correlation between ‘Lee index’ and carcass fat content in weanling and adult female rats with hypothalamic lesions. J Endocrinol 1968; 40: 527-528
• 24 Taylor BA, Phillips SJ. Detection of obesity QTLs on mouse chromosomes 1 and 7 by selective DNA pooling. Genomics 1996; 34: 389-398
• 25 Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972; 18: 499-502
• 26 Cacho J, Sevillano J, de Castro J et al. Validation of simple indexes to assess insulin sensitivity during pregnancy in Wistar and Sprague-Dawley rats. Am J Physiol Endocrinol Metab 2008; 295: E1269-E1276
• 27 Dobiasova M, Frohlich J. The plasma parameter log (TG/HDL-C) as an atherogenic index: correlation with lipoprotein particle size and esterification rate in apo-B lipoprotein-depleted plasma (FER (HDL)). Clin Biochem 2001; 34: 583-588
• 28 Alladi S, Shanmugasundaram KR. Induction of hypercholesterolaemia by supplementing soy protein with acetate generating amino acids. Nutr Rep Int 1989; 40: 893-900
• 29 Nepokroeff CM, Lakshmanan MR, Porter JW. Fatty-acid synthase from rat liver. Methods Enzymol 1975; 35: 37-44
• 30 Noltmann EA, Gubler CJ, Kuby SA. Glucose-6-phosphate dehydrogenase (Zwischenferment). I. Isolation of the crystalline enzyme from yeast. J Biol Chem 1961; 236: 1225-1230
• 31 da Silva Mattos AM, Xavier CH, Karlen-Amarante M et al. Renal sympathetic nerve activity is increased in monosodium glutamate induced hyperadipose rats. Neurosci Lett 2012; 522: 118-122
• 32 Nakagawa T, Ukai K, Ohyama T et al. Effects of chronic administration of sibutramine on body weight, food intake and motor activity in neonatally monosodium glutamate-treated obese female rats: relationship of antiobesity effect with monoamines. Exp Anim 2000; 49: 239-249
• 33 Lazarin Mde O, Ishii-Iwamoto EL, Yamamoto NS et al. Liver mitochondrial function and redox status in an experimental model of non-alcoholic fatty liver disease induced by monosodium L-glutamate in rats. Exp Mol Pathol 2011; 91: 687-694
• 34 Rotimi OA, Olayiwola IO, Ademuyiwa O et al. Effects of fibre-enriched diets on tissue lipid profiles of MSG obese rats. Food Chem Toxicol 2012; 50: 4062-4067
• 35 Ansari JA. Protective role of Zingiber officinale to curb visceral obesity induced by monosodium glutamate on neonatal Wistar rats. J Obes Weight Loss Ther 2013; 3: 7
• 36 Rol de Lama MA, Perez-Romero A, Hermanussen M et al. Sexual dimorphism in growth as measured by microknemometry: different responses to GH deficiency and exogenous GH administration. Neuroendocrinology 1998; 68: 210-219
• 37 Yamazaki RK, Brito GA, Coelho I et al. Low fish oil intake improves insulin sensitivity, lipid profile and muscle metabolism on insulin resistant MSG-obese rats. Lipids Health Dis 2011; 10: 66
• 38 Balbo SL, Grassiolli S, Ribeiro RA et al. Fat storage is partially dependent on vagal activity and insulin secretion of hypothalamic obese rat. Endocrine 2007; 31: 142-148
• 39 Guimaraes RB, Telles MM, Coelho VB et al. Adrenalectomy abolishes the food-induced hypothalamic serotonin release in both normal and monosodium glutamate-obese rats. Brain Res Bull 2002; 58: 363-369
• 40 Dolnikoff M, Martin-Hidalgo A, Machado UF et al. Decreased lipolysis and enhanced glycerol and glucose utilization by adipose tissue prior to development of obesity in monosodium glutamate (MSG) treated-rats. Int J Obes Relat Metab Disord 2001; 25: 426-433
• 41 Zhang WM, Kuchár S, Mozes S. Body fat and RNA content of the VMH cells in rats neonatally treated with monosodium glutamate. Brain Res Bull 1994; 35: 383-385
• 42 Martinková A, Lenhardt L, Mozes S. Effect of neonatal MSG treatment on day-night alkaline phosphatase activity in the rat duodenum. Physiol Res 2000; 49: 339-345
• 43 Srichamroen A, Field CJ, Thomson ABR et al. The modifying effects of galactomannan from Canadian-grown fenugreek (Trigonella foenum-graecum L.) on the glycemic and lipidemic status in rats. J Clin Biochem Nutr 2008; 43: 167-174
• 44 Kobayashi H, Hirabayashi Y, Murakami H et al. Anti-obesity effects of amino acid in high-fat diet induced obese mice. FASEB J 2009; 23: 227.5
• 45 Vijayakumar MV, Pandey V, Mishra GC et al. Hypolipidemic effect of fenugreek seeds is mediated through inhibition of fat accumulation and upregulation of LDL receptor. Obesity (Silver Spring) 2010; 18: 667-674
• 46 Scalbert A, Johnson IT, Saltmarsh M. Polyphenols: antioxidants and beyond. Am J Clin Nutr 2005; 81 (1 Suppl) 215S-217S
• 47 Banda SDT, Suneetha D, Reddy TNVS. In-vivo anti-oxidant activity of methanolic extract of Sapindus emarginatus in monosodium glutamate induced obesity rats. Int J Pharm & Life Sci 2013; 4: 3105-3108
• 48 Suneetha D, Banda SDT, Ali F. Antiobesity values of methanolic extract of Sapindus emariganatus on monosodium glutamate induced model in rats. IJPPR 2013; 5: 267-270
• 49 Jeyakumar SM, Lopamudra P, Padmini S et al. Fatty acid desaturation index correlates with body mass and adiposity indices of obesity in Wistar NIN obese mutant rat strains WNIN/Ob and WNIN/GR-Ob. Nutr Metab (Lond) 2009; 6: 27
• 50 Yoshioka K, Yoshida T, Kondo M. Reduced brown adipose tissue thermogenesis and metabolic rate in pre-obese mice treated with monosodium-L-glutamate. Endocrinol Jpn 1991; 38: 75-79
• 51 Guilherme A, Virbasius JV, Puri V et al. Adipocyte dysfunctions linking obesity to insulin resistance and type 2 diabetes. Nat Rev Mol Cell Biol 2008; 9: 367-377
• 52 Reaven GM. Why Syndrome X? From Harold Himsworth to the insulin resistance syndrome. Cell Metab 2005; 1: 9-14
• 53 Rolo AP, Palmeira CM. Diabetes and mitochondrial function: role of hyperglycemia and oxidative stress. Toxicol Appl Pharmacol 2006; 212: 167-178
• 54 Havel PJ. Update on adipocyte hormones: regulation of energy balance and carbohydrate/lipid metabolism. Diabetes 2004; 53: S143-S151
• 55 Hamza N, Berke B, Cheze C et al. Preventive and curative effect of Trigonella foenum-graecum L. seeds in C57BL/6J models of type 2 diabetes induced by high-fat diet. J Ethnopharmacol 2012; 142: 516-522
• 56 Chang CJ, Tzeng TF, Liou SS et al. Regulation of lipid disorders by ethanol extracts from Zingiber zerumbet in high-fat diet-induced rats. Food Chem 2012; 132: 460-467
• 57 Ansell BJ, Watson KE, Fogelman AM et al. High-density lipoprotein function recent advances. J Am Coll Cardiol 2005; 46: 1792-1798
• 58 Holzwarth-McBride MA, Sladek Jr JR, Knigge KM. Monosodium glutamate induced lesions of the arcuate nucleus. II. Fluorescence histochemistry of catecholamines. Anat Rec 1976; 186: 197-205
• 59 Aizawa-Abe M, Ogawa Y, Masuzaki H et al. Pathophysiological role of leptin in obesity-related hypertension. J Clin Invest 2000; 105: 1243-1252
• 60 de Melo CL, Queiroz MG, Arruda Filho AC et al. Betulinic acid, a natural pentacyclic triterpenoid, prevents abdominal fat accumulation in mice fed a high-fat diet. J Agric Food Chem 2009; 57: 8776-8781
• 61 Cao H, Gerhold K, Mayers JR et al. Identification of a lipokine, a lipid hormone linking adipose tissue to systemic metabolism. Cell 2008; 134: 933-944
• 62 Salati LM, Amir-Ahmady B. Dietary regulation of expression of glucose-6-phosphate dehydrogenase. Annu Rev Nutr 2001; 21: 121-140
• 63 Wakil SJ, Abu-Elheiga LA. Fatty acid metabolism: target for metabolic syndrome. J Lipid Res 2009; 50 (Suppl) S138-S143
• 64 Schleinitz D, Kloting N, Korner A et al. Effect of genetic variation in the human fatty acid synthase gene (FASN) on obesity and fat depot-specific mRNA expression. Obesity (Silver Spring) 2010; 18: 1218-1225