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Thromb Haemost 2002; 88(02): 274-281
DOI: 10.1055/s-0037-1613198
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Schattauer GmbH

Ciglitazone Inhibits Plasmin-Induced Proinflammatory Monocyte Activation via Modulation of p38 MAP Kinase Activity

Tatiana Syrovets
1   Department of Pharmacology of Natural Products and Clinical Pharmacology, University of Ulm, Germany
,
Almut Schüle
1   Department of Pharmacology of Natural Products and Clinical Pharmacology, University of Ulm, Germany
,
Marina Jendrach
1   Department of Pharmacology of Natural Products and Clinical Pharmacology, University of Ulm, Germany
,
Berthold Büchele
1   Department of Pharmacology of Natural Products and Clinical Pharmacology, University of Ulm, Germany
,
Thomas Simmet
1   Department of Pharmacology of Natural Products and Clinical Pharmacology, University of Ulm, Germany
› Author Affiliations
Further Information

Publication History

Received 30 November 2001

Accepted after revision 30 April 2002

Publication Date:
07 December 2017 (online)

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Summary

Plasmin triggers chemotaxis and NF-κBand AP-1-mediated proinflammatory gene expression in human peripheral monocytes (PM). Compared with macrophages and dendritic cells, PM express mainly the peroxisome proliferator-activated receptor (PPAR) γ and traces of PPARα as detected by semiquantitative RT-PCR and immunoblotting. The PPARγ agonist ciglitazone, but not the PPARα agonist clofibric acid, concentration-dependently inhibited the plasmin-, but not the FMLP-induced PM chemotaxis. Similarly, release of interleukin (IL)-1α, IL-1β and tumor necrosis factor (TNF)-α from plasmin-stimulated PM was concentration-dependently inhibited by ciglitazone, but not by clofibric acid, while the LPS-induced TNF-α release remained unaffected by any of both PPAR agonists. Ciglitazone activates PPARγ as shown by a novel surface plasmon resonance analysis and inhibits the plasmin-induced activation of NF-κB and AP-1. It also inhibits p38 MAPK phosphorylation essential for the plasmin-induced PM chemotaxis and gene activation. Thus, activation of PPARγ by ciglitazone may allow controling of the plasmin-mediated recruitment and activation of PM at sites of inflammation.

Keywords

Monocytes/macrophages - cytokines - chemotaxis - transcription factors - p38 MAPK
 

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