A rapid, automated flow cytometric method to measure activated degranulated platelets by density determination

Sabrinah E. Chapman; Martin Sorette; Edward Hetherington; David Zelmanovic; Gail Kling; Jacques Dugailliez; Nuria Pujol-Moix; David Okrongly

doi:10.1055/s-0037-1613402

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2003; 89(06): 1004-1015
DOI: 10.1055/s-0037-1613402

Platelets and Blood Cells

Schattauer GmbH

A rapid, automated flow cytometric method to measure activated degranulated platelets by density determination

Sabrinah E. Chapman

¹Bayer Diagnostics, New York, U.S.A

,

Martin Sorette

¹Bayer Diagnostics, New York, U.S.A

,

Edward Hetherington

¹Bayer Diagnostics, New York, U.S.A

,

David Zelmanovic

¹Bayer Diagnostics, New York, U.S.A

,

Gail Kling

¹Bayer Diagnostics, New York, U.S.A

,

Jacques Dugailliez

¹Bayer Diagnostics, New York, U.S.A

,

Nuria Pujol-Moix

¹Bayer Diagnostics, New York, U.S.A

,

David Okrongly

¹Bayer Diagnostics, New York, U.S.A

› Institutsangaben

Abstract

Summary

Platelet activation is reported to correlate with acute coronary syndromes. A platelet analysis method on the ADVIA^®120 Hematology System provides rapid analysis of platelet density, reported as mean platelet component (MPC) concentration, utilizes routine hematology specimens, requires no pre-treatment, and thirty seconds to generate results. Sub-populations of platelets separated by density gradients showed excellent correlation with the ADVIA 120 MPC parameter (r = 0.997). Platelet activation induced by thrombin treatment resulted in a shift of platelets into the lowest density fraction (d ≤ 1.068 g/mL) with a corresponding reduction in MPC from 24.7 to 20.6 g/dL, N = 4 subjects (p < 0.004). There was also excellent correlation between expression of CD62P measured by fluorescence flow cytometry and the ADVIA 120 Hematology System MPC values (r = 0.85). These results indicate that the ADVIA 120 MPC parameter may be a useful new test for assessing activated platelets and platelet density.

Keywords

Platelets - activation - density - CD62P - ADVIA 120

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Referenzen

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