The Influence Exerted by a Restricted Phospholipid Microenvironment on the Expression of Tissue Factor Activity at the Cell Plasma Membrane Surface

Corinne Kunzelmann-Marche; Nathalie Satta; Florence Toti; Youming Zhang; Peter P. Nawroth; James H. Morrissey; Jean-Marie Freyssinet

doi:10.1055/s-0037-1613800

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2000; 83(02): 282-289
DOI: 10.1055/s-0037-1613800

Rapid Communication

Schattauer GmbH

The Influence Exerted by a Restricted Phospholipid Microenvironment on the Expression of Tissue Factor Activity at the Cell Plasma Membrane Surface

Corinne Kunzelmann-Marche

¹From the Institut d’Hématologie et d’Immunologie, Faculté de Médecine, Université Louis Pasteur, Strasbourg

²Unité 143 INSERM, Hôpital de Bicêtre, Le Kremlin-Bicêtre, France

,

Nathalie Satta

¹From the Institut d’Hématologie et d’Immunologie, Faculté de Médecine, Université Louis Pasteur, Strasbourg

²Unité 143 INSERM, Hôpital de Bicêtre, Le Kremlin-Bicêtre, France

⁴Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA

,

Florence Toti

¹From the Institut d’Hématologie et d’Immunologie, Faculté de Médecine, Université Louis Pasteur, Strasbourg

²Unité 143 INSERM, Hôpital de Bicêtre, Le Kremlin-Bicêtre, France

,

Youming Zhang

³Department of Internal Medicine IV, Section Vascular Medicine, Tübingen, Germany

,

Peter P. Nawroth

³Department of Internal Medicine IV, Section Vascular Medicine, Tübingen, Germany

,

James H. Morrissey

⁴Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA

,

Jean-Marie Freyssinet

¹From the Institut d’Hématologie et d’Immunologie, Faculté de Médecine, Université Louis Pasteur, Strasbourg

²Unité 143 INSERM, Hôpital de Bicêtre, Le Kremlin-Bicêtre, France

› Institutsangaben

Abstract

Summary

Phosphatidylserine (PhtdSer) is an anionic aminophospholipid necessary for the development of optimal tissue factor (TF) activity at the cell surface. This study investigates the implication of a restricted lipid environment with respect to PhtdSer availability on TF expression and activity. K562 cells, showing a reduced ability to externalize PhtdSer, were transfected with human TF cDNA. PhtdSer exposure and TF activity were examined in transfected cells and compared to monocytic THP-1 cells expressing constitutive and inducible TF or megakaryocytic HEL cells showing a high PhtdSer externalization potency. TF expression was evidenced by flow cytometry and its activity measured using functional assays. PhtdSer exposure was monitored by enzymatic prothrombinase assay. One clone (DC9) expressed a stable amount of TF antigen without global modification of its membrane status. Despite a noticeable TF expression level, clone DC9 presented only a weak TF activity even after ionophore stimulation. The apparent K_m , relative to factor X (FX) activation by TF-factor VIIa (FVIIa) complex, was 335 nM versus 70 nM for THP-1 cells. The velocity of the reaction was found 3-fold slower in DC9 than THP-1 cells. Ionophore treatment resulting in slightly enhanced amounts of available PhtdSer abolished this difference. The DC9 clone appears suitable for further investigations on the biology of TF expressed at the surface of cells where the contribution of PhtdSer is significantly attenuated. Such cells should enable further assessment of the role of TF as a receptor coupled to intracellular signaling pathways and its fate during apoptotic cell death.

Abbreviations: TF: tissue factor, FX: factor X, FVII(a): factor VII(a), PhtdSer: phosphatidylserine, PhtdCho: phosphatidylcholine, PhtdEth: phosphatidylethanolamine, BSA: bovine serum albumin, dcTF: des-cyto tissue factor (TF_1-244: membrane-anchored TF lacking the cytoplasmic tail)

Keywords

Tissue factor - phosphatidylserine - K562 cells - procoagulant activity - microparticle release

Volltext

Referenzen

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