Presence of Two Plasminogen Alleles in Normal Populations

Masafumi Kida; Masuyo H.- Kawabata; Tomio Yamazaki; Akitada Ichinose

doi:10.1055/s-0037-1614235

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1998; 79(01): 150-154
DOI: 10.1055/s-0037-1614235

Review Article

Schattauer GmbH

Presence of Two Plasminogen Alleles in Normal Populations

Masafumi Kida

¹The Department of Molecular Pathological Biochemistry, Yamagata University School of Medicine, Yamagata, Japan

,

Masuyo H.- Kawabata

²The Department of Biochemistry, University of Washington, Seattle, WA, USA

,

Tomio Yamazaki

¹The Department of Molecular Pathological Biochemistry, Yamagata University School of Medicine, Yamagata, Japan

,

Akitada Ichinose

¹The Department of Molecular Pathological Biochemistry, Yamagata University School of Medicine, Yamagata, Japan

²The Department of Biochemistry, University of Washington, Seattle, WA, USA

› Author Affiliations

Abstract

Summary

When we compared nucleotide sequences of the 5’-flanking regions for plasminogen genes from 11 individuals, six substitutions were identified even among normal subjects. A new haplotype (termed allele II) was screened by PCR-RFLP analysis among 54 Japanese and 58 Caucasian normal subjects. The frequency of allele II was 0.787 in the Japanese and 0.560 in the Caucasians, indicating that the ratio of alleles differs between populations. Examination of 118 cases with dysplasminogenemia revealed that the Ala601-Thr mutation was present on allele I in most cases. This mutation was also associated with allele II in one-fourth of all cases, suggesting possible recombination within the plasminogen gene. Interestingly, we previously demonstrated that the expression activity of allele II was about 1.8 fold that of allele I in vitro.

Full Text

References

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