Fibrillin Containing Elastic Microfibrils Support Platelet Adhesion under Dynamic Shear Conditions

Julia M. Ross; Larry V. McIntire; Joel L. Moake; Huey-Ju Kuo; Rui-Qing Qian; Robert W. Glanville; Elaine Schwartz; Jacob H. Rand

doi:10.1055/s-0037-1614236

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1998; 79(01): 155-161
DOI: 10.1055/s-0037-1614236

Review Article

Schattauer GmbH

Fibrillin Containing Elastic Microfibrils Support Platelet Adhesion under Dynamic Shear Conditions

Julia M. Ross^*

¹From the Cox Laboratory for Biomedical Engineering, Rice University, Houston, TX, USA

,

Larry V. McIntire

¹From the Cox Laboratory for Biomedical Engineering, Rice University, Houston, TX, USA

,

Joel L. Moake

¹From the Cox Laboratory for Biomedical Engineering, Rice University, Houston, TX, USA

²From the Division of Hematology, Baylor College of Medicine, Houston, TX, USA

,

Huey-Ju Kuo

³From the Shriners Hospital for Crippled Children, Portland, OR, USA

,

Rui-Qing Qian

³From the Shriners Hospital for Crippled Children, Portland, OR, USA

,

Robert W. Glanville

³From the Shriners Hospital for Crippled Children, Portland, OR, USA

,

Elaine Schwartz

⁴From the Department of Dermatology, Mt. Sinai School of Medicine, New York, NY, USA

,

Jacob H. Rand

⁵From the Hematology Division, Mt. Sinai School of Medicine, New York, NY, USA

› Author Affiliations

Abstract

Summary

The vascular subendothelium contains macromolecular structures called microfibrils. Type VI collagen is one protein found in microfibrils that supports platelet adhesion and aggregation and we have previously evaluated the roles of platelet receptors and vWf involved in these processes under physiological shear conditions. Here we investigate the ability of fibrillin containing elastic microfibrils to support mural thrombus formation. Our results show that elastic microfibril surfaces support platelet adhesion under low shear conditions at a level similar to collagen VI tetramers. However, the degree of aggregation on the elastic microfibril surface is much higher. Both adhesion and aggregation were shown to be mediated by the GPIIb-IIIa platelet receptor. Elastic microfibrils do not support the formation of mural thrombi under high shear conditions. These results suggest roles for both collagen VI and fibrillin containing elastic microfibrils in modulating the platelet response to blood vessel injury.

Full Text

References

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