Thrombopoietin Activates Human Platelets and Induces Tyrosine Phosphorylation of p80/85 Cortactin

Michaëla Fontenay-Roupie; Guillaume Huret; Jean-Pierre Loza; Raphaël Adda; Josiane Melle; Jacques Maclouf; François Dreyfus; Sylviane Lévy-Toledano

doi:10.1055/s-0037-1614239

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1998; 79(01): 195-201
DOI: 10.1055/s-0037-1614239

Review Article

Schattauer GmbH

Thrombopoietin Activates Human Platelets and Induces Tyrosine Phosphorylation of p80/85 Cortactin

Michaëla Fontenay-Roupie

¹From the Laboratoire d’Hématologie, Hôpital Cochin, Paris

²From the INSERM U363, ICGM, Université Paris V, Hôpital Cochin, Paris

,

Guillaume Huret

¹From the Laboratoire d’Hématologie, Hôpital Cochin, Paris

,

Jean-Pierre Loza

¹From the Laboratoire d’Hématologie, Hôpital Cochin, Paris

,

Raphaël Adda

¹From the Laboratoire d’Hématologie, Hôpital Cochin, Paris

,

Josiane Melle

¹From the Laboratoire d’Hématologie, Hôpital Cochin, Paris

,

Jacques Maclouf

³From the INSERM U348, Institut Fédératif de Recherche Circulation, Hôpital Lariboisière, Paris, France

,

François Dreyfus

¹From the Laboratoire d’Hématologie, Hôpital Cochin, Paris

²From the INSERM U363, ICGM, Université Paris V, Hôpital Cochin, Paris

,

Sylviane Lévy-Toledano

³From the INSERM U348, Institut Fédératif de Recherche Circulation, Hôpital Lariboisière, Paris, France

› Institutsangaben

Abstract

Summary

The mechanism of human platelet activation by thrombopoietin (TPO) was investigated in vitro. We found that rHuTPO stimulated thromboxane A2 formation and serotonin secretion, despite the absence of shape change and aggregation. Blockade of the arachidonic acid pathway did not inhibit rHuTPO-induced platelet secretion. rHuTPO stimulated the tyrosine phosphorylation of 64, 80/85, 95, 130 and 140 kDa proteins, but phosphoproteins of 100-105 and 125 kDa obtained when platelets aggregated in the presence of thrombin were absent. rHuTPO stimulated and potentiated the thrombin-induced tyrosine phosphorylation of a 80 kDa protein identified as the cortical actin-associated protein, p80/85 cortactin. When platelets were aggregated in the presence of rHuTPO and fibrinogen, cortactin phosphorylation was enhanced as compared to rHuTPO alone. Treatment with RGDS or cytochalasin D respectively reduced or abolished cortactin tyrosine phosphorylation. This confirms the existence of fibrinogen binding-dependent and independent pools of phosphorylated cortactin, both requiring intact actin polymerization. Cytoskeleton-binding proteins may be implicated in in vitro platelet activation by rHuTPO.

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