Platelet Compatibility of an Artificial Surface Modified with Functionally Active Heparin

Tom Eirik Mollnes; Vibeke Videm; Dorte Christiansen; Grethe Bergseth; Johan Riesenfeld; Torstein Hovig

doi:10.1055/s-0037-1614342

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1999; 82(03): 1132-1136
DOI: 10.1055/s-0037-1614342

Letters to the Editor

Schattauer GmbH

Platelet Compatibility of an Artificial Surface Modified with Functionally Active Heparin

Authors

Tom Eirik Mollnes

¹From the Department of Immunology and Transfusion Medicine, Nordland Central Hospital, and University of Tromsø, Stockholm, Sweden
Vibeke Videm

²Department of Immunology and Blood Bank, Norwegian University of Science and Technology, Trondheim, Sweden
Dorte Christiansen

¹From the Department of Immunology and Transfusion Medicine, Nordland Central Hospital, and University of Tromsø, Stockholm, Sweden
Grethe Bergseth

¹From the Department of Immunology and Transfusion Medicine, Nordland Central Hospital, and University of Tromsø, Stockholm, Sweden
Johan Riesenfeld

⁴Carmeda AB, Stockholm, Sweden
Torstein Hovig

³Department of Pathology, The National Hospital, University of Oslo, Norway

Abstract

Summary

Platelet compatibility after coating an artificial material with functionally active heparin was investigated. Blood was circulated in uncoated or heparin coated PVC tubing. In one hour platelet counts decreased from 155 (113-184) ×10⁹/l to 124 (100-148) ×10⁹/l with uncoated compared to 164 (132-192) ×10⁹/l with heparin coated tubing (intergroup p = 0.02). β-thromboglobulin increased from 116 (80-148) μg/l to 1039 (757-1298) μg/l with uncoated and to 352 (229-638) μg/l with heparin coated tubing (intergroup p = 0.005). Platelet counts and β-thromboglobulin correlated closely with complement activation. Solid-phase enzyme immunoassay demonstrated substantial deposition of CD42a/GPIbIX and CD61/GPIIIa on uncoated, but not on heparin coated tubing (intergroup p <0.0005). Scanning electron microscopy demonstrated activated platelets and aggregates on uncoated in contrast to heparin coated tubing, where scattered, unactivated platelets were found. Changes in P-selectin and microparticles were minor. In conclusion, this heparin surface substantially improved platelet compatibility. Markers of choice for in vitro evaluation were platelet counts, β-thromboglobulin and platelet deposition.

Key words

Platelets - heparin - complement - artificial surface - β-thromboglobulin - P-selectin - microparticles - flow cytometry - biocompatibility

Full Text

References

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