Effects of Endotoxemia on Thrombopoiesis in Men

Petra Stohlawetz; Claudia C. Folman; Albert E. G. Kr. von dem Borne; Thomas Pernerstorfer; Hans-Georg Eichler; Simon Panzer; Bernd Jilma

doi:10.1055/s-0037-1614534

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1999; 81(04): 613-617
DOI: 10.1055/s-0037-1614534

Rapid Communication

Schattauer GmbH

Effects of Endotoxemia on Thrombopoiesis in Men

Petra Stohlawetz

²Clinic of Blood Group Serology and Transfusion Medicine, Transfusion Medicine, Vienna University School of Medicine, Vienna, Austria

,

Claudia C. Folman

³Department of Hematology, Div. of Internal Medicine Academic Medical Center, University of Amsterdam, Central Laboratory of Experimental and Clinical Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands

,

Albert E. G. Kr. von dem Borne

³Department of Hematology, Div. of Internal Medicine Academic Medical Center, University of Amsterdam, Central Laboratory of Experimental and Clinical Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands

,

Thomas Pernerstorfer

¹From the Departments of Clinical Pharmacology, the Adhesion Research Group Elaborating Therapeutics, Vienna, Austria

,

Hans-Georg Eichler

³Department of Hematology, Div. of Internal Medicine Academic Medical Center, University of Amsterdam, Central Laboratory of Experimental and Clinical Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands

,

Simon Panzer

²Clinic of Blood Group Serology and Transfusion Medicine, Transfusion Medicine, Vienna University School of Medicine, Vienna, Austria

,

Bernd Jilma

¹From the Departments of Clinical Pharmacology, the Adhesion Research Group Elaborating Therapeutics, Vienna, Austria

› Author Affiliations

Abstract

Summary

Background. Febrile conditions are often associated with increased platelet turnover and refractoriness to platelet transfusions, although several pyrogenic cytokines enhance thrombopoiesis. This study aimed to characterize the effects of experimental human endotoxemia on platelet turnover and thrombopoiesis. Methods. Endotoxin (4 ng/kg) was infused into 30 healthy men to study the regulation of thrombopoiesis in systemic human inflammation. Platelet counts, plasma thrombopoietin (TPO) and glycocalicin levels, and reticulated platelets (RP) were measured to evaluate the effect of acute endotoxemia on thrombopoiesis. Ten subjects received pretreatment with 1000 mg aspirin po. to evaluate possible effects of aspirin on platelet turnover, and ten subjects received paracetamol to control for effects of anti-pyresis. Results. Platelet counts dropped by about 15% (p <0.001) one hour after LPS infusion, began to recover at 24 h, and exceeded baseline values by 8% (CI: 5-12; p <0.001) at 7 days after LPS iv. Reticulated platelet counts increased from 1.62% (CI: 1.24-2.0) to a maximum of 2.39% (CI: 1.81-2.98; p = 0.003) at 6 h. TPO levels increased from baseline values of 10 A.U/ml (CI: 8.8-11.2) to 15.5 A.U/ml (CI: 13.6-17.3) at 24 h (p <0.001), whereas plasma glycocalicin was not changed (p >0.05). The number of circulating platelet-neutrophil aggregates increased more than 100% at 6 h (p <0.001). Neither aspirin nor paracetamol affected changes in any of the parameters measured. Conclusion. Low grade endotoxemia induces a rapid fall of platelet counts, which is followed by an early increase in reticulated platelets and TPO levels but not of glycocalicin levels. Finally peripheral platelet counts increase several days after LPS infusion.

Full Text

References

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