Regulation of Monocyte Tissue Factor Activity by Allogeneic and Xenogeneic Endothelial Cells

Christoph W. Kopp; Simon C. Robson; Jonathan B. Siegel; Josef Anrather; Hans Winkler; Shane Grey; Elzbieta Kaczmarek; Fritz H. Bach; Carolyn L. Geczy

doi:10.1055/s-0037-1614939

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1998; 79(03): 529-538
DOI: 10.1055/s-0037-1614939

Review Articles

Schattauer GmbH

Regulation of Monocyte Tissue Factor Activity by Allogeneic and Xenogeneic Endothelial Cells

Christoph W. Kopp

¹From the Sandoz Center for Immunobiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA; and The Heart Research Institute, Camperdown, Australia

,

Simon C. Robson

¹From the Sandoz Center for Immunobiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA; and The Heart Research Institute, Camperdown, Australia

,

Jonathan B. Siegel

¹From the Sandoz Center for Immunobiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA; and The Heart Research Institute, Camperdown, Australia

,

Josef Anrather

¹From the Sandoz Center for Immunobiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA; and The Heart Research Institute, Camperdown, Australia

,

Hans Winkler

¹From the Sandoz Center for Immunobiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA; and The Heart Research Institute, Camperdown, Australia

,

Shane Grey

¹From the Sandoz Center for Immunobiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA; and The Heart Research Institute, Camperdown, Australia

,

Elzbieta Kaczmarek

¹From the Sandoz Center for Immunobiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA; and The Heart Research Institute, Camperdown, Australia

,

Fritz H. Bach

¹From the Sandoz Center for Immunobiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA; and The Heart Research Institute, Camperdown, Australia

,

Carolyn L. Geczy

¹From the Sandoz Center for Immunobiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA; and The Heart Research Institute, Camperdown, Australia

› Author Affiliations

Abstract

Summary

The regulation of tissue factor (TF) activity by the cell associated tissue factor pathway inhibitor (TFPI) during monocyte (Mo) and endothelial cell (EC) interactions is not fully understood. This report describes co-ordinate induction of TF antigen (TF-Ag) and membrane-associated TFPI-Ag on human Mo following coculture with human aortic (HAEC) or porcine aortic EC (PAEC) or after stimulation with TNFα. We show that both allo- and xenogeneic EC induce human Mo-TF antigen in short-term culture. However, the TF activity of TNFα-primed Mo is suppressed when these cells are cocultured with HAEC [by 40.3 ± 6.3% (p <0.02)] or PAEC [by 50.5 ± 10.6% (p <0.001)]. Antibody (Ab) blocking studies confirm that TFPI is the principal anticoagulant associated with this suppression of TF-activity. Our data indicate that anti-TF activity originates, at least in part, from the activated human Mo in the coculture; additionally, specific generation of TFPI by Mo is observed under the xenogeneic culture conditions. As Mo associated TF, induced by allo- or xenogeneic EC interactions, is regulated by cell-associated TFPI, we propose that infiltrating Mo may modulate the thrombotic process at sites of vascular injury in association with both allo- and xenograft rejection.

Aspects of the work were presented at the American Society for Hematology meeting in Orlando, FL, December 1996.

Full Text

References

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