Cell-derived Microparticles Circulate in Healthy Humans and Support Low Grade Thrombin Generation

René J. Berckmans; Rienk Nieuwland; Anita N. Böing; Fred P. H. T. M. Romijn; C. Erik Hack; Augueste Sturk

doi:10.1055/s-0037-1615646

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2001; 85(04): 639-649
DOI: 10.1055/s-0037-1615646

Review Articles

Schattauer GmbH

Cell-derived Microparticles Circulate in Healthy Humans and Support Low Grade Thrombin Generation

René J. Berckmans

¹Department of Clinical Chemistry, Leiden University Medical Center, Leiden

,

Rienk Nieuwland

¹Department of Clinical Chemistry, Leiden University Medical Center, Leiden

,

Anita N. Böing

¹Department of Clinical Chemistry, Leiden University Medical Center, Leiden

,

Fred P. H. T. M. Romijn

¹Department of Clinical Chemistry, Leiden University Medical Center, Leiden

,

C. Erik Hack

²Central Laboratory of the Netherlands Red Cross Bloodtransfusion Service, Sanquin Blood Supply Foundation and University Hospital Vrije Universiteit, Amsterdam, The Netherlands

,

Augueste Sturk

¹Department of Clinical Chemistry, Leiden University Medical Center, Leiden

› Author Affiliations

Abstract

Summary

We determined the numbers, cellular origin and thrombin-generating properties of microparticles in healthy individuals (n = 15). Micro-particles, isolated from fresh blood samples and identified by flow cytometry, originated from platelets [23710⁶/L (median; range 116-565)], erythrocytes (2810⁶/L; 13-46), granulocytes (4610⁶/L; 16-94) and endothelial cells (6410⁶/L; 16-136). They bound annexin V, indicating surface exposure of phosphatidylserine, and supported coagulation in vitro. Interestingly, coagulation occurred via tissue factor (TF)-independent pathways, because antibodies against TF or factor (F)VII were ineffective. In contrast, in our in vitro experiments coagulation was partially inhibited by antibodies against FXII (12%, p = 0.006), FXI (36%, p <0.001), FIX (28%, p <0.001) or FVIII (32%, p <0.001). Both the number of annexin V-positive microparticles present in plasma and the thrombin-generating capacity inversely correlated to the plasma concentrations of thrombin-antithrombin complex (r = -0.49, p = 0.072 and r = -0.77, p = 0.001, respectively), but did not correlate to prothrombin fragment F₁₊₂ (r = -0.002, p = 0.99). The inverse correlations between the number of microparticles and their thrombin-forming capacity and the levels of thrombin-antithrombin complex in plasma may indicate that microparticles present in the circulation of healthy individuals have an anticoagulant function by promoting the generation of low amounts of thrombin that activate protein C. We conclude that microparticles in blood from healthy individuals support thrombin generation via TF- and FVII-independent pathways, and which may have an anticoagulant function.

Keywords

Microparticles - coagulation - thrombin generation - flow cytometry - tissue factor

Full Text

References

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