Serotonin 5-HT1 Receptors Potentiate Histamine and Thrombin Stimulated Prostaglandin Synthesis in Endothelial Cells

Edward J. Kilbourne; Richard C. Winneker

doi:10.1055/s-0037-1615769

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2001; 85(05): 924-928
DOI: 10.1055/s-0037-1615769

Review Article

Schattauer GmbH

Serotonin 5-HT₁ Receptors Potentiate Histamine and Thrombin Stimulated Prostaglandin Synthesis in Endothelial Cells

Edward J. Kilbourne

¹Endocrinology Division, Women’s Health Research Institute, Wyeth Ayerst Research, Radnor, PA, USA

,

Richard C. Winneker

¹Endocrinology Division, Women’s Health Research Institute, Wyeth Ayerst Research, Radnor, PA, USA

› Author Affiliations

Abstract

Summary

The ability of serotonin 5-HT₁ receptors to increase vascular tone was previously found to be activated by vasoconstrictiors such as hista-mine. In this study, treatment of cultured human aortic endothelial cells (HAEC) with the 5-HT₁-selective agonist 5-carboxamidotryptamine (5-CT) alone had no effect on the levels of prostaglandin F_2α (PGF_2α) or 6-keto-prostaglandin F_1α (6-keto PGF_α1). However, 5-CT potentiated the histamine and thrombin stimulated increases in prostaglandins released by HAEC. In the presence of histamine, increasing doses of 5-CT caused a steep rise in PGF_2α levels resulting in an increase in the ratio of PGF_2α over 6-keto PGF_1α . The ability of 5-CT to potentiate prostaglandin production was correlated with its ability to potentiate the histamine and thrombin mediated mobilization of arachidonic acid. These results demonstrate that the ability of 5-HT₁ receptors to stimulate prostaglandin production in endothelial cells is activated by histamine and thrombin.

Keywords

Endothelial cells - serotonin - histamine - prostaglandin

Full Text

References

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