Fluorescence and Cerenkov luminescence imaging

Summary

This review addresses small animal optical imaging (OI) applications in diverse fields of basic research. In the past, OI has proven to be cost- and time-effective, allows real-time imaging as well as high-throughput analysis and does not imply the usage of ionizing radiation (with the exception of Cerenkov imaging applications). Therefore, this technique is widely spread – not only geographically, but also among very different fields of basic research – and is represented by a large body of publications. Originally used in oncology research, OI is nowadays emerging in further areas like inflammation and infectious disease as well as neurology. Besides fluorescent probe-based contrast, the feasibility of Cerenkov luminescence imaging (CLI) has been recently shown in small animals and thus represents a new route for future applications.

Thus, this review will focus on examples for OI applications in inflammation, infectious disease, cell tracking as well as neurology, and provides an overview over CLI.

Zusammenfassung

Diese Übersicht befasst sich mit verschiedenen Anwendungsgebieten der optischen Bildgebung im Bereich der Grundlagenforschung an Kleintieren. Die optische Bildgebung hat sich als eine kosten- und zeitgünstige Alternative zu anderen bildgebenden Modalitäten erwiesen, sie ermöglicht das Verfolgen von biologischen Prozessen in vivo in Echtzeit sowie Hochdurchsatzanalysen und impliziert nicht notwendigerweise die Verwendung ionisierender Strahlung – mit Ausnahme der Cerenkov-Lumineszenz-Bildgebung. Weiterhin ist die optische Bildgebung geografisch und in verschiedensten Fachrichtungen in der Anwendung sehr weit verbreitet. Ursprünglich vor allem in der onkologischen Grundlagenforschung eingesetzt, findet die optische Bildgebung mittlerweile auch in anderen Forschungsgebieten wie beispielsweise bei entzündlichen, infektiösen oder neurologischen Krankheiten Anwendung. Neben der Fluoreszenz-Bildgebung wird neuerdings die Cerenkov-Lumineszenz als neues Kontrastierungsverfahren in der Kleintierforschung verwendet.

Keywords

Optical imaging - small animal - fluorescence - Cerenkov luminescence

Schlüsselwörter

Optische Bildgebung - Kleintier - Fluoreszenz - Cerenkov-Lumineszenz

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