A presumptive case of cerebral babesiosis in a dog in Poland caused by a virulent Babesia canis strain

L. Adaszek; M Górna; P. Klimiuk; M Kalinowski; S. Winiarczyk

doi:10.1055/s-0038-1623660

Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere, Table of Contents

Tierarztl Prax Ausg K Kleintiere Heimtiere 2012; 40(05): 367-371
DOI: 10.1055/s-0038-1623660

Case Report

Schattauer GmbH

A presumptive case of cerebral babesiosis in a dog in Poland caused by a virulent Babesia canis strain

Ein vermutlicher Fall von zerebraler Babesiose bei einem Hund in Polen verursacht durch einen virulenten Babesia-canis-Stamm

L. Adaszek

¹Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland

,

M Górna

¹Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland

,

P. Klimiuk

²Department of Pathological Anatomy, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland

,

M Kalinowski

¹Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland

,

S. Winiarczyk

¹Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland

› Author Affiliations

Abstract

Summary

The aim of this paper was to present the first case of cerebral canine babesiosis due to infection by Babesia canis in a dog in Poland. A 5-year-old American Staffordshire Terrier was presented with an unusual clinical manifestation of acute babesiosis that included neurological signs and pancytopenia. Despite treatment the dog died. Diagnosis was based on microscopic examination of Giemsa-stained blood smears (detection of piroplasms in red blood cells) and post mortem examination of the brain by histopathology and PCR method. The amplified segment of the Babesia 18S RNA gene was sequenced. This enabled to determine that the cause of the disease had been the strain 18S RNA-B EU622793. This is one of two B. canis strains found endemically in Poland, which reveals a greater virulence than the strain 18S RNA-A EU622792. The described case indicates that this form of canine babesiosis should be taken into account in differential diagnosis in dogs exhibiting neurological symptoms, especially in the tick activity season.

Zusammenfassung

Der Fallbericht beschreibt den ersten Fall einer durch Babesia canis verursachten zerebralen Babesiose bei einem Hund in Polen. Ein 5 Jahre alter American Staffordshire Terrier wurde mit ungewöhnlicher klinischer Manifestation einer akuten Babesiose in Form von neurologischen Symptomen und Panzytopenie vorgestellt. Trotz Behandlung starb der Hund. Die Diagnose basierte auf der mikroskopischen Untersuchung nach Giemsa gefärbter Blutausstriche (Nachweis von Piroplasmen in Erythrozyten) und den Befunden der histopathologischen und mittels PCR-Verfahren durchgeführten Untersuchung des Gehirns postmortal. Das mittels PCR amplifizierte Fragment des Babesia-18S-RNA-Gens wurde sequenziert. Dies ermöglichte die Identifikation des Babesia-canis-Stamms 18S RNA-B EU622793 als Ursache der Erkrankung. Hierbei handelt es sich um einen von zwei in Polen endemisch vorkommenden B.-canis-Stämmen, der eine größere Virulenz aufweist als der Stamm 18S RNA-A EU622792. Der Fall zeigt, dass diese Form der Babesiose bei Hunden mit neurologischen Symptomen differenzialdiagnostisch berücksichtigt werden sollte, vor allem in der Saison der Zeckenaktivität.

Key words

Babesia canis - cerebral babesiosis - dog - PCR - vector-borne diseases

Schlüsselwörter

Babesia canis - zerebrale Babesiose - Hund - PCR - vektorübertragene Krankheiten

Full Text

References

References
1 Adaszek L, Winiarczyk S. Molecular characterization of Babesia canis canis isolates from naturally infected dogs in Poland. Vet Parasitol 2008; 152: 235-241.
2 Adaszek L, Winiarczyk S, Skrzypczak M. The clinical course of babesiosis in 76 dogs infected with protozoa parasites Babesia canis canis. Pol J Vet Sci 2009; 12: 81-87.
3 Adaszek L, Twaróg D, Zietek J, Wojciechowski M, Winiarczyk S. Comparison of standard PCR and Real-Time PCR in the diagnose of canine parvoviral infections. Medycyna Weter 2009; 65: 683-686.
4 Adaszek L, Winiarczyk S, Lukaszewska J. A first case of ehrlichiosis in a horse in Poland. Dtsch Tieraerztl Wochenschr 2009; 116: 330-334.
5 Adaszek L, Winiarczyk S. Application of the SYBR Green real-time HRM PCR technique in the differentiation of the Babesia canis canis protozoa isolated in the areas of eastern Poland. Parasitol Res 2010; 106: 1253-1256.
6 Adaszek L, Winiarczyk S, Górna M. From piroplasmosis to babesiosis – problems with classification of Babesia protozoa isolated from dogs. Wiad Parazytol 2010; 56: 111-115.
7 Adaszek L, Martinez AC, Winiarczyk S. The factors affecting the distribution of babesiosis in dogs in Poland. Vet Parasitol 2011; 181: 160-165.
8 Ajayi SA. A survey of cerebral babesiosis in Nigerian local cattle. Vet Rec 1978; 103: 564.
9 Bonawitz C, Castillo M, Mukherji K. Comparison of CT and MR features with clinical outcome in patients with rocky mountain spotted fever. Am J Neuroradiol 1997; 18: 459-464.
10 Boozer AL, Macintire DK. Canine babesiosis. Vet Clin North Am Small Anim Pract 2003; 33: 885-904.
11 Cardoso L, Costa A, Tuna J, Vieira L, Eyal O, Yisaschar-Mekuzas Y, Baneth G. Babesia canis canis and Babesia canis vogeli infections in dogs from northern Portugal. Vet Parasitol 2008; 156: 199-204.
12 Carret C, Walas F, Carcy B, Grande N, Précigout É, Moubri K, Schetters T, Gorenflot A. Babesia canis canis, Babesia canis vogeli, Babesia canis rossi: Differentiation of three subspecies by restriction fragment length polymorphism analysis on amplified small subunit ribosomal RNA genes. J Euk Microbiol 1999; 46: 298-303.
13 Chaturvedi U, Tiwari AK, Ratta B, Ravindra PV, Rajawat YS, Palia SK, Rai A. Detection of canine adenoviral infections in urine and faeces by the polymerase chain reaction. J Virol Methods 2008; 149: 260-263.
14 De Vos AJ, Imes GD, Cullen JS. Cerebral babesiosis in a new-born calf. Onderstepoort J Vet Res 1976; 43 (02) 75-78.
15 Evans HE, de Lahunta A. Guide to the Dissection of the Dog. 7th. edn. Philadelphia: Saunders; 2009
16 Gothe R, Wegerdt S. Die Babesiosen des Hundes in Deutschland: epidemiologische Fallanalysen. Tierärztl Prax 1991; 19: 170-173.
17 Irwin PJ. Canine babesiosis: from molecular taxonomy to control. Parasit Vectors 2009; (Suppl. 01) S4.
18 Jacobson LS, Clark IA. The pathophysiology of canine babesiosis: new approaches to an old puzzle. J S Afr Vet Assoc 1994; 65: 134-145.
19 Jacobson LS. The South African form of severe and complicated canine babesiosis: clinical advances 1994-2004. Vet Parasitol 2006; 138: 126-139.
20 Kubelová M, Tkadlec E, Bednár M, Roubalová E, Siroký P. West-to-east differences of Babesia canis canis prevalence in Dermacentor reticulatus ticks in Slovakia. Vet Parasitol 2011; 180: 191-196.
21 Malherbe D. The manifestations and diagnosis of Babesia infections. Ann New York Acad Sci 1956; 64: 128-146.
22 Máthé A, Vörös K, Papp L, Reiczigel J. Clinical manifestations of canine babesiosis in Hungary (63 cases). Acta Vet Hung 2006; 54: 367-385.
23 Matjila PT, Penzhorn BL, Bekker CP, Nijhof AM, Jongejan F. Confirmation of occurrence of Babesia canis vogeli in domestic dogs in South Africa. Vet Parasitol 2004; 122: 119-125.
24 Okoh AE. A case of cerebral babesiosis in the dog. Bull Anim Health Prod Afr 1978; 26: 118-119.
25 Patankar TF, Karnad DR, Shetty PG, Desai AP, Prasad SR. Adult cerebral malaria: Prognostic importance of imaging findings and correlation with postmortem findings. Radiology 2002; 224: 811-816.
26 Schetters TP, Eling WM. Can Babesia infections be used as a model for cerebral malaria?. Parasitol Today 1999; 15: 492-497.
27 Shaw SE, Day MJ, Birtles RJ, Breitschwerdt EB. Tick-borne infectious diseases of dogs. Trends Parasitol 2001; 17: 74-80.
28 Solano-Gallego L, Trotta M, Carli E, Carcy B, Caldin M, Furlanello T. Babesia canis canis and Babesia canis vogeli clinicopathological findings and DNA detection by means of PCR-RFLP in blood from Italian dogs suspected of tick-borne disease. Vet Parasitol 2008; 157: 211-221.
29 Solano-Gallego L, Baneth G. Babesiosis in dogs and cats – expanding parasitological and clinical spectra. Vet Parasitol 2011; 181: 48-60.
30 Taboada J, Babesiosis Greene CE. eds. Infectious Diseases of the Dog and Cat. 2nd. edn. Philadelphia: Saunders; 1998: 473-481.
31 Tarasów E, Ustymowicz A, Zajkowska J, Hermanowska-Szpakowicz T. Neuroborreliosis: CT and MRI findings in 14 cases. Preliminary communication. Neurolog Neurochir Pol 2001; 35: 803-813.
32 Van de Maele I, Savary-Bataille K, Gielen I, Daminet S. An unusual form of canine babesiosis. Can Vet J 2008; 49: 283-286.
33 Zahler M, Schein E, Rinder H, Gothe R. Characteristic genotypes discriminate between Babesia canis isolates of differing vector specificity and pathogenicity in dogs. Parasitol Res 1998; 84: 544-548.
34 Zygner W, Rapacka G, Gójska-Zygner O, Dlugosz E, Wedrychowicz H. Biochemical abnormalities observed in serum of dogs infected with large Babesia in Warsaw (Poland). Pol J Vet Sci 2007; 10: 245-253.