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Nuklearmedizin 1989; 28(03): 105-109
DOI: 10.1055/s-0038-1629479
Original Articles
Schattauer GmbH

Iron-Binding Proteins and 67Ga Accumulation by Tumor Cells

L. J. Anghileri
1   From the Nuclear Medicine Service and Biophysics Laboratory, Faculty of Medicine, University of Nancy, France
,
P. Thouvenot
1   From the Nuclear Medicine Service and Biophysics Laboratory, Faculty of Medicine, University of Nancy, France
,
Marie-Christine Crone-Escanye
1   From the Nuclear Medicine Service and Biophysics Laboratory, Faculty of Medicine, University of Nancy, France
,
F. Brunotte
1   From the Nuclear Medicine Service and Biophysics Laboratory, Faculty of Medicine, University of Nancy, France
,
J. Robert
1   From the Nuclear Medicine Service and Biophysics Laboratory, Faculty of Medicine, University of Nancy, France
› Author AffiliationsThe authors thank Professor Dr. Klaus Goerttler, Institut für Experimentelle Pathologie, Deutsches Krebsforschungszentrum, Heidelberg, FRG, for the tumors used in this work.
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Publication History

Received: 19 December 1988

Publication Date:
04 February 2018 (online)

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Tumor cells incubated with 67Ga-citrate plus lactoferrin showed a lactoferrin concentration-dependent increase of 67Ga uptake while for transferrin the effect was an inhibition. A correlation was observed between the binding of 131l-labelled lactoferrin or 131l-labelled transferrin and the uptake of 67Ga by the cells. Saturation with iron did not change significantly the effects of the ironbinding proteins. Preincubation with lactoferrin, transferrin or ferric citrate increased 67Ga uptake. The formation of a ternary complex in which 67Ga mimics iron is discussed.

Zusammenfassung

Tumorzellen, die mit 67Ga-Zitrat und Laktoferrin inkubiert wurden, zeigten eine Zunahme der 67Ga-Anreicherung in Abhängigkeit von der Laktoferrin-Konzentration, während mit Transferrin ein inhibitorischer Effekt eintrat. Eine Korrelation wurde zwischen der Bindung von 131I-markiertem Laktoferrin oder 131I-markiertem Transferrin mit der Anreicherung von 67Ga durch die Zellen beobachtet. Die Sättigung mit Eisen veränderte die Wirkungen der eisenbindenden Proteine nicht signifikant. Die vorherige Inkubation mit Laktoferrin, Transferrin oder Eisenzitrat erhöhte die 67Ga-Anreicherung. Die Bildung eines ternären Komplexes, in dem 67Ga Eisen simuliert, wird diskutiert.

 

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