Download PDF
Thromb Haemost 1973; 29(02): 461-469
DOI: 10.1055/s-0038-1647786
Original Article
Schattauer GmbH

Neuraminidase Injections in Rabbits

Reduced Platelet Surface Charge, Aggregation and Thrombocytopenia
A. K Grøttum
1   Section of Haematology (Chief, Professor P. F. Hjort), Medical Department A (Chief: Professor E. Gjone), Rikshospitalet, Oslo, Norway
,
M Jeremic
1   Section of Haematology (Chief, Professor P. F. Hjort), Medical Department A (Chief: Professor E. Gjone), Rikshospitalet, Oslo, Norway
› Author Affiliations
Further Information

Publication History

Received for publication 24 September 1972

Publication Date:
30 June 2018 (online)

Also available at
    Permissions and Reprints

Summary

Two types of neuraminidase from CL perfringens were injected intravenously in rabbits. Blood platelet electrophoretic mobility was markedly reduced 4 hours after the injection of the enzyme. A marked thrombocytopenia developed gradually with a mean lower level of 55 and 27% of the preinjection number 48 hours after the injec- - tion. White blood cell count and factors V and VIII were reduced.

The results indicate that rabbit platelets with a reduced surface charge5 due to cleavage of sialic acid from the platelet membrane, are not immediately aggregated, trapped or destroyed in vivo. We suggest that the enzyme-induced platelet membrane changes rendered the platelets more susceptible to destruction in the reticuloendothelial system with appearance of thrombocytopenia after some hours.

 

  • References

  • 1 Abramson H. 1965. Electrophoresis of blood cells. In: Sawyer P. N. (ed.), Biophysical mechanisms in vascular homeostasis and intravascular thrombosis . Appleton-Century-Crofts; New York: 3.
    Search in Google Scholar
  • 2 Ada G. L, Stone J. D. 1950; Electrophoretic studies on virus-red cell interaction: Mobility gradient of cells treated with viruses of the influenza group and the receptor-destroying enzyme of Vibrio cholerae. British Journal of Experimental Pathology 31: 263.
    PubMedSearch in Google Scholar
  • 3 Baddini M. 1966; Idiopathic thrombocytopenic purpura. New England Journal of Medicine 274: 1245.
    CrossrefPubMedSearch in Google Scholar
  • 4 Blombäck B, Blombäck M. 1956; Purification of human and bovine fibrinogen. Arkiv for Kemi 10: 415.
    PubMedSearch in Google Scholar
  • 5 Brecher G, Schneideman M, Cronkite E. P. 1953; The reproducibility and constancy of the platelet count. American Journal of Clinical Pathology 23: 15.
    CrossrefPubMedSearch in Google Scholar
  • 6 Choi, Sun-I Simone J. V, Journey L. J. 1972; Neuraminidase-induced thrombocytopenia in rats. British Journal of Haematology 22: 93.
    CrossrefPubMedSearch in Google Scholar
  • 7 Cohen P, Braunwald J, Gardner F. H. 1965; Destruction of canine and rabbit platelets following intravenous administration of carbon particles and endotoxin. Journal of Laboratory and Clinical Medicine 66: 263.
    PubMedSearch in Google Scholar
  • 8 Cook G. M. W, Heard D. H, Seaman G. V. F. 1961; Sialic acids and the electrokinetic charge of the human erythrocytes. Nature 191: 44.
    CrossrefPubMedSearch in Google Scholar
  • 9 Eylar E. H, Madoff M. A, Brody O. V, Oncley J. L. 1962; The contribution of sialic acid to the surface charge of the erythrocytes. Journal of Biological Chemistry 237: 1992.
    PubMedSearch in Google Scholar
  • 10 Furuhjelm U, Myllylä G, Nevanlinna H. R, Nordling S, Pirkola A, Gavin J, Gooch A, Sanger R, Tippett P. 1969; The red cell phenotype En(a) and anti-Ena: Serological and physicochemical aspects. Vox Sanguinis 17: 256.
    PubMedSearch in Google Scholar
  • 11 Gasig G. J, Gasig T. B, Stewart C. C. 1968; Antimetastatic effects associated with platelet reduction. Proceedings of the National Academy of Sciences of the United States of America (Washington) 61: 46.
    CrossrefPubMedSearch in Google Scholar
  • 12 Godal H. C. 1961; Simple syneresis procedure for fibrinogen assay. Scandinavian Journal of Clinical and Laboratory Investigation 13: 530.
    CrossrefPubMedSearch in Google Scholar
  • 13 Grøttum K. A. 1968; Influence of aggregating agents on electrophoretic mobility of blood platelets from healthy individuals and from patients with cardiovascular diseases. Lancet I: 1406.
    PubMedSearch in Google Scholar
  • 14 Geøttum K. A, Solum N. O. 1969; Congenital thrombocytopenia with giant platelets : a defect in the platelet membrane. British Journal of Haematology 16: 277.
    CrossrefPubMedSearch in Google Scholar
  • 15 Grotttjm K. A. 1969; Platelet surface charge and aggregation. Effects of polyelectrolytes. Thrombosis et Diathesis Haemorrhagica 21: 450.
    PubMedSearch in Google Scholar
  • 16 Grotttjm K. A, Hjort P. F, Jeremic M. 1969; Interaction of bacterial endotoxin and Liquoid with blood platelets : Aggregation and decrease in electrokinetic charge. Thrombosis et Diathesis Haemorrhagica 22: 192.
    PubMedSearch in Google Scholar
  • 17 Grøttum K. A, Jorgensen L, Jeremic M. 1972; Decrease in platelet surface charge during phagocytosis of polystyrene latex particles or thorium dioxide. Scandinavian Journal of Haematology 09: 83.
    PubMedSearch in Google Scholar
  • 18 Hanig M. 1948; Electrokinetic charge in human erythrocytes during adsorption and elution of PR 8 influenza virus. Proceedings of the Society of Experimental Biology 68: 385.
    PubMedSearch in Google Scholar
  • 19 Hovig T. 1965; Effects of various enzymes on the ultrastructure, aggregation, and clot retraction ability of rabbit blood platelets. Thrombosis et Diathesis Haemorrhagica 13: 84.
    PubMedSearch in Google Scholar
  • 20 Jerushalmy Z, Kohn A, de Vries A. 1961; Interaction of myxoviruses with human blood platelets in vitro. Proceedings of the Society of Experimental Biological Medicine 106: 462.
    PubMedSearch in Google Scholar
  • 21 Madoff M. A, Ebbe S, Baldini M. 1964; Sialic acid of human blood platelets. Journal of Clinical Investigation 43: 870.
    CrossrefPubMedSearch in Google Scholar
  • 22 Mester L. 1971. Platelet sialoglycoproteins. In: Caen J. (ed.), Platelet aggregation. Masson & Cie . Paris: 131.
    Search in Google Scholar
  • 23 Schiffman S, Rapaport S. I, Patch M. J. 1963; The identification and synthesis of activated plasma thromboplastin component (PTC). Blood 22: 733.
    PubMedSearch in Google Scholar
  • 24 Stormorken H. 1957; The preparation of proaccelerin deficient (Parahemophilic) plasma for the assay of proaccelerin. Scandinavian Journal of Clinical and Laboratory Investigation 09: 273.
    CrossrefPubMedSearch in Google Scholar
  • 25 Terada H, Baldini M, Ebbe S, Madoff M. A. 1966; Interaction of influenza virus with blood platelets. Blood 28: 213.
    CrossrefPubMedSearch in Google Scholar
  • 26 Weed R. I. 1970; The importance of erythrocyte deformability. American Journal of Medicine 49: 147.
    CrossrefPubMedSearch in Google Scholar
  • 27 Weiss L. 1965; Studies on cell deformability. I. Effect of surface charge. Journal of Cellular Biology 26: 735.
    CrossrefPubMedSearch in Google Scholar