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Thromb Haemost 1973; 29(03): 572-578
DOI: 10.1055/s-0038-1647798
Original Article
Schattauer GmbH

The Determination of Optimum Glycine Concentration for the Preparation of Human Fibrinogen at Ambient Temperatures

Edward B. Silberstein
1   Radioisotope Laboratory Department of Radiology and Division of Hematology, Department of Medicine, University of Cincinnati Medical Center
,
Samuel C. Ingraham III
1   Radioisotope Laboratory Department of Radiology and Division of Hematology, Department of Medicine, University of Cincinnati Medical Center
,
James G. Kereiakes
1   Radioisotope Laboratory Department of Radiology and Division of Hematology, Department of Medicine, University of Cincinnati Medical Center
› Author Affiliations
Further Information

Publication History

Publication Date:
24 July 2018 (online)

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Summary

A glycine-precipitation method is presented allowing the preparation of human fibrinogen of high clottability and stability at ambient temperature (25 ± 3° C) with a minimum of specialized equipment. From barium sulfate adsorbed plasma, the optimum yield of clottable fibrinogen is found to occur with a 65% relative glycine saturation of plasma.

 

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