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Thromb Haemost 1975; 33(02): 335-340
DOI: 10.1055/s-0038-1647885
Original Article
Schattauer GmbH

Heterogeneity of Rabbit Platelets

IV. Thrombocytosis with Absolute Megathrombocytosis in Phenylhydrazine-induced Hemolytic Anemia in Rabbits
Michael L Freedman
1   Department of Medicine, New York University Medical School, 550 First Avenue, New York, NY 10016
,
Simon Karpatkin
1   Department of Medicine, New York University Medical School, 550 First Avenue, New York, NY 10016
› Author Affiliations
Further Information

Publication History

Received 22 October 1974

Accepted 02 January 1975

Publication Date:
02 July 2018 (online)

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Summary

Intravenous injection of Phenylhydrazine into intact rabbits resulted in a thrombocytosis of 1.85 fold and megathrombocytosis (large platelets) of 2.30 fold. Splenectomy markedly impaired this response. The intravenous injection of red blood cell hemo-lysates gave a response similar to that of Phenylhydrazine in both intact and splenec-tomized animals. Basal data obtained from intact and splenectomized animals; as well as kinetic data obtained following apparent splenic blockade secondary to Phenylhydrazine hemolysis revealed the presence of a splenic platelet pool with preferential splenic sequestration of megathrombocytes.

It is suggested that the thrombocytosis and absolute megathrombocytosis of hemolysis is due to splenic blockade by red blood cell hemolysate.

 

  • References

  • 1 Brecher G, Cronkite E.P. Morphology and enumeration of human blood platelets. Journal of Applied Physiology 1950; 3: 365
    CrossrefPubMedSearch in Google Scholar
  • 2 Charmatz A, Karpatkin S. Heterogeneity of rabbit platelets 1. Employment of an albumin density gradient for separation of a young platelet population identified with Se75selenomethionine. Thrombosis et Diathesis Haemorrhagica 1974; 31: 485
    PubMedSearch in Google Scholar
  • 3 Chen L. T, Weiss L. The role of the sinus wall in the passage of erythrocytes through the spleen. Blood 1973; 41: 529
    PubMedSearch in Google Scholar
  • 4 Ebbe S, Baldini M, Donovan J. Comparative studies of platelet survival by different methods in the rabbit. Blood 1965; 25: 548
    PubMedSearch in Google Scholar
  • 5 Garg S. K, Amorosi E. L, Karpatkin S. Use of the megathrombocyte as an index of megakaryocyte number. New England Journal of Medicine 1971; 284: 11
    CrossrefPubMedSearch in Google Scholar
  • 6 Karpatkin S, Garg S. K, Siskind G. W. Autoimmune thrombocytopenic purpura and the compensated thrombocytolytic state. American Journal of Medicine 1971; 51: 1
    CrossrefPubMedSearch in Google Scholar
  • 7 Shulman N. R, Watkins Jr. S. P, Itscoitz S. B, Students A. B. Evidence that the spleen retains the youngest and hemostatically most effective platelets. Transactions of the Association of American Physicians 1968; 81: 302
    PubMedSearch in Google Scholar
  • 8 Weiner M, Karpatkin S. Use of the megathrombocyte to demonstrate thrombopoietin. Thrombosis et Diathesis Haemorrhagica 1972; 28: 24
    PubMedSearch in Google Scholar
  • 9 Williams W. J. Thrombocytosis. In: Williams W. J, Beutler E, Erslev A. J, Rundles R. W. (eds.) Hematology, McGraw-Hill; New York: 1972: 1163
    Search in Google Scholar