Increased Protein Synthesis by Human Platelets during Phagocytosis of Latex Particles in Vitro

Hana Bessler; Galila Agam; Meir Djaldetti

doi:10.1055/s-0038-1647928

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1976; 35(02): 350-357
DOI: 10.1055/s-0038-1647928

Original Article

Schattauer GmbH

Increased Protein Synthesis by Human Platelets during Phagocytosis of Latex Particles in Vitro

Authors

Hana Bessler

¹Department of Medicine “B” and Hematology Clinic, Hasharon Hospital, Petah-Tiqva, and Tel-Aviv University Medical School, Israel
Galila Agam

¹Department of Medicine “B” and Hematology Clinic, Hasharon Hospital, Petah-Tiqva, and Tel-Aviv University Medical School, Israel
Meir Djaldetti^*

¹Department of Medicine “B” and Hematology Clinic, Hasharon Hospital, Petah-Tiqva, and Tel-Aviv University Medical School, Israel

Abstract

Summary

A three-fold increase of protein synthesis by human platelets during in vitro phagocytosis of polystyrene latex particles was detected. During the first two hours of incubation, the percentage of phagocytizing platelets and the number of latex particles per platelet increased; by the end of the third hour, the first parameter remained stable, while the number of latex particles per cell had decreased.

Vincristine (20 μg/ml of cell suspension) inhibited platelet protein synthesis. This effect was both time- and dose-dependent. The drug also caused a decrease in the number of phagocytizing cells, as well as in their phagocytotic activity.

Volltext

Referenzen

References
1 Behnke O. 1968; Electron microscopical observations on the surface coating of human platelets. Journal of Ultrastructure Research 24: 51.
2 Boesen E, Davis W. 1969. Cytotoxic drug in the treatment of cancer. Edward Arnold; London: 151.
3 Clawson C. C, White J. G. 1971; Platelet interaction with bacteria. II. Fate of the bacteria. American Journal of Pathology 65: 381.
4 Cook G. M. W. 1970. Role of glycoprotein cell membrane. FEBF Summer School; University of Helsinki:
5 Creasey W. A, Markiw M. E. 1964; Biochemical effects of the vinca alkaloids. II. A comparison of the effect of colchicine, vinblastine and vincristine on the synthesis of ribonucleic acids in Ehrlich ascites carcinoma cells. Biochimica et Biophysica Acta (Amst.) 87: 601.
6 Creasey W. A, Markiw M. E. 1965; Biochemical effects of vinca alkaloids. III. The synthesis of nucleic acid and the incorporation of amino acids in Ehrlich ascites cell in vitro. Biochimica et Biophysica Acta (Amst.) 103: 635.
7 Danon D, Jerusalmy Z, de Vries A. 1959; Incorporation of influenza virus in human blood platelets in vitro. Electron microscopic observation. Virology 9: 719.
8 Dejesus M, Fiklrig S, Detwiler T. 1972; Phagocytosis - stimulated NBT reduction by platelets. Journal of Laboratory and Clinical Medicine 80: 111.
9 Elsbach P. 1967; Stimulation of lecithin synthesis from medium lysolecithin during phagocytosis. Journal of Clinical Investigation 46: 1052.
10 Frei III E, Whang J, Scoggins R. B. 1964; The stathmokinetic effect of vincristine. Cancer Research 24: 1918.
11 Glynn M. F, Movat H. J, Murphy E. A, Mustard J. F. 1965; Study of platelet adhesiveness and aggregation with latex particles. Journal of Laboratory and Clinical Medicine 65: 179.
12 Glynn M. F, Herren R, Mustard J. F. 1966; Adherence of latex particles to platelets. Nature (London) 212: 19.
13 Grøttum K. A, Jogensen L, Jeremic M. 1972; Decrease in platelet surface charge during phagocytosis of polystyrene latex particles or thorium dioxide. Scandinavian Journal of Haematology 9: 83.
14 Hoving R. 1968; The ultrastructure of blood platelets in normal and abnormal states. Series Haematologica 1: 3.
15 Karnovsky M. L, Wallach D. F. H. 1961; The metabolic basis of phagocytosis. III. Incorporation of inorganic phosphate into various classes of phosphatides during phagocytosis. Journal of Biological Chemistry 236: 1895.
16 Kuramoto A, Steiner M, Baldini M. G. 1970; Metabolic basis of platelet phagocytosis. Biochimica et Biophysica Acta 201: 471.
17 Lampkin B. C, Mc Williams N. B, Mauer A. M. 1972; Cell kinetics and chemotherapy in acute leukemia. Seminars in Hematology 9: 211.
18 Mani M. J, Firkin B. G. 1972; Uptake of latex and thorotrast by human platelets in vitro: effect of various chemicals demonstrating differing mechanisms and metabolic requirements. British Journal of Haematology 22: 383.
19 Marmont A. M, Damasio E. E. 1967; The effect of two alkaloids derived from Vinka rosea on the malignant cells of Hodjkin’s disease. lymphosarcoma and acute leukemia in vivo. Blood 29: 1.
20 Movat H. Z, Weiser W. J, Glynn M. F, Mustard J. F. 1965; Platelets phagocytosis and aggregation. Journal of Cell Biology 27: 531.
21 Mustard J. F, Packham M. A. 1968; Platelet phagocytosis. Series Haematologica 1: 168.
22 Rak K. 1972; Effect of vincristine on platelet production in mice. British Journal of Haematology 22: 617.
23 Shirasawa K, Chandler A. B. 1969; Fine structure of the bond between platelets in artificial thrombi and platelet aggregates induced by adenosine diphosphate. American Journal of Pathology 57: 127.
24 Stehbens W. E, Biscoe T. J. 1967; The ultrastructure of early platelet aggregation in vivo . American Journal of Pathology 50: 219.
25 Van Furth R. 1970. Mononuclear phagocytes. 1st ed.. Blackwell Scientific Publications; Oxford and Edinburgh: 212.
26 White J. G. 1968; Effect of colchicine and vinka alkaloids on human platelets. II. Changes in the dense tubular system and formation of unusual inclusion in incubated cells. American Journal of Pathology 53: 447.
27 White J. G. 1972; Uptake of latex particles by blood platelets phagocytosis or sequestration. American Journal of Pathology 69: 439.