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DOI: 10.1055/s-0038-1648023
Platelet Preservation. II. The Response of Human Platelet Suspensions to Hypotonic Stress
Publication History
Received
06 November 1975
Accepted
26 February 1976
Publication Date:
03 July 2018 (online)
Summary
1. Platelet suspensions were exposed to hypotonic stress. Several parameters of the changes in light absorbance were investigated i. a. the initial decrease in absorbance (Amin), the maximal rate of the recovery process (Vmax), and the value of the absorbance two hours after mixing the platelet suspension with the hypotonic solution.
2. The ratio Vmax/Amin appeared to be independent of both platelet concentration and, within a specific range, decrease in osmolarity.
3. Cryoprotectants appeared to disturb the response to hypotonic stress.
4. Cryopreservation caused a decrease in the light absorbance of the platelet suspension, of Amin, and of the recovery process.
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References
- 1 Baserga A, Ballerini G. 1957; La résistance osmotique plaquettaire dans différentes conditions hématologiques. Schweizerische Medizinische Wochenschrift 87: 1218.
- 2 Born G. V. R. 1972. Platelets. Functional physiology. In: Biggs R. (ed.), Human Blood Coagulation, Haemostasis and Thrombosis.. Blackwell Scientific Publications; Oxford, London, Edinburgh, Melbourne: 159.
- 3 Fantl P. 1968; Osmotic stability of blood platelets. Journal of Physiology 198: 1.
- 4 Handin R.L, Fortier N. L, Valeri C. R. 1970; Platelet response to hypotonic stress after storage at 4 C or 22 C. Transfusion 10: 305.
- 5 Hardeman M. R. 1975; The effect of continuous dialysis on platelet preservation. Transfusion 15: 231.
- 6 Hardeman M. R, Heynens C. J. L. 1974a The active serotonin uptake and the hypotonic shock response of human blood platelets. Methods, normal values and the influence of various experimental conditions. Thrombosis et Diathesis Haemorrhagica 32: 391.
- 7 Hardeman M. R, Heynens C. J. L. 1974b Storage of human blood platelets. The serotonin uptake and hypotonic shock response as in vitro viability tests. Thrombosis et Diathesis Haemorrhagica 32: 405.
- 8 Kahn R. A. 1973; Alteration in optical density of platelets exposed to hypertonic solutions. Proceedings of the Society for Experimental Biology and Medicine 143: 1094.
- 9 Kim B. K, Baldini M. G. 1973; Preservation of viable platelets by freezing. Effect of plastic containers. Proceedings of the Society for Experimental Biology and Medicine 142: 345.
- 10 Kim B. K, Baldini M. G. 1974a Biochemistry, function and hemostatic effectiveness of frozen human platelets. Proceedings of the Society for Experimental Biology and Medicine 145: 830.
- 11 Kim B. K, Baldini M. G. 1974b The platelet response to hypotonic shock. Its value as an indicator of platelet viability after storage. Transfusion 14: 130.
- 12 Odink J, Sprokholt R. 1975 Platelet preservation. I. The influence of cooling conditions and cryoprotectants on human platelets. Submitted for publication.
- 13 Rogner G. 1963; Die Bestimmung der osmotischen Thrombozytenresistenz mit dem Elektrophotometer. Acta Haematologia 29: 346.
- 14 Sixma J. J, Linssen W. H, Geuze H. J. 1972; Gluteraldehyde fixation of human blood platelets. Thrombosis et Diathesis Haemorrhagica 27: 272.
- 15 Valeri C. R, Feingold H, Marchionni L. D. 1974; The relation between response to hypotonic stress and the 51Cr recovery in vivo of preserved platelets. Transfusion 14: 331.