Some Observations on the Preparation of Platelet-Rich Plasma

Brian P. Woods; Angela Dennehy; Noel Clarke

doi:10.1055/s-0038-1648045

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1976; 36(02): 302-310
DOI: 10.1055/s-0038-1648045

Original Article

Schattauer GmbH

Some Observations on the Preparation of Platelet-Rich Plasma

Brian P. Woods^*

¹Department of Pathology, University College, Earlsfort Terrace, Dublin 2, Ireland

,

Angela Dennehy

¹Department of Pathology, University College, Earlsfort Terrace, Dublin 2, Ireland

,

Noel Clarke

¹Department of Pathology, University College, Earlsfort Terrace, Dublin 2, Ireland

› Author Affiliations

Abstract

Summary

In order to prepare platelet-rich plasma a constant height and volume of citrated whole blood was centrifuged at different gravity forces and times. The supernatant plasmas were examined for platelet concentrations, plasma volumes and optical densities. Results are described showing that gravity force and time have an important influence on the platelet numbers and the plasma volumes recovered.

The light attenuation by different concentrations of platelets in plasma, using light path lengths of 5 mm and 10 mm, was compared. The shorter light path provides greater linearity between optical density and platelet numbers especially at higher concentrations.

Full Text

References

References
1 Born G. V. R, Cross M. J. 1963; The aggregation of blood platelets. Journal of Physiology 168: 178.
2 Chaplin H, Mollison P. L. 1952; Correction for plasma trapped in the red cell column of the hematocrit. Blood 7: 1227.
3 Clarke N, Moriarty T, Woods B. P. 1972; An apparatus for producing and monitoring platelet aggregation in whole blood. Irish Journal of Medical Science 141: 61.
4 Clarke N, McCabe V. 1973; The Model II aggregometer for producing and monitoring platelet aggregation in whole blood. Proceedings of the IVth International Congress on Thrombosis and Haemostasis, Vienna, 19-22 June 1973: 103.
5 Clarke N, McCabe V, Woods B. P, Dennehy A, Moriarty T. 1975; Preliminary observations on the technique of monitoring platelet aggregation in whole blood using a rotor system. Irish Journal of Medical Science 144: 116.
6 Corn M, Pozderac R. 1967; Estimation of platelet count from optical density of platelet-rich plasma. Blood 30: 545.
7 Cruz W. O. 1954; Platelet determination by turbidimetry. Blood 9: 920.
8 Day H. J, Hardisty R. M. 1974; Report of the task force on platelet standards. Thrombosis et Diathesis Haemorrhagica suppl 59: 307.
9 De Duve C, Berthet J, Beaufay H. 1959; Gradient centrifugation of cell particles: theory and applications. Progress in Biophysical and Biochemical Chemistry 9: 325.
10 Frojmovic M. M. 1973; Quantitative parameterization of the light transmission properties of citrated, platelet-rich plasma as a function of platelet and adenosine diphosphate concentrations and temperature. Journal of Laboratory and Clinical Medicine 82: 137.
11 Kerker M. 1969. The Scattering of Light. Academic Press; London.:
12 McBride J. A. 1968; Platelet adhesiveness: the effect of centrifugation on the measurement of platelet adhesiveness in platelet-rich plasma. Journal of Clinical Pathology 21: 397.
13 McCabe V, Clarke N. 1973; Optical density of plasma as a function of platelet concentration, wavelength of light and optical geometry of spectrophotometer. Proceedings of the IVth International Congress on Thrombosis and Haemostasis, Vienna, 19–22 June 1973, 133.
14 McCabe V, Clarke N. 1975; A computer model for migration of platelets during the preparation of platelet-rich plasma from whole blood. Proceedings of the International Symposium on Research and Clinical Applications in Haemodynamics, Dublin, 23–25 September 1975, 48.
15 Maupin B. 1969. Blood Platelets in Man and Animals. Pergamon Press; London.:
16 Moriarty T, McCabe V, Clarke N. 1975; Details of a rotor system for investigating platelet aggregation in whole blood. Irish Journal of Medical Science 144: 203.
17 Newcombe J. F. 1964; Differential plasma turbidity as an index of platelet counts. Nature 204: 87.
18 O’Brien J. R. 1962; Platelet aggregation. II. Some results from a new method of study. Journal of Clinical Pathology 15: 452.
19 O’Brien J. R. 1970; Factors influencing the optical platelet aggregation test. Acta Medica Scandinavica suppl 525: 43.
20 Stormorken H, Lund-Riise Aa, Rørvik T. O. 1965; Platelet adhesiveness to glass beads; methodological investigations using automatic platelet counting. Scandinavian Journal of Clinical and Laboratory Investigation 17 suppl. 84: 183.
21 Woods B. P, Clarke N. 1971; Standardisation in the preparation of platelet-rich plasma; the relationship between platelet numbers and optical density. Proceedings of the IInd International Congress on Thrombosis and Haemostasis, Oslo, 12–16 July 1971, 272.
22 Woods B. P. 1973. Studies on plasma platelet suspensions; their preparation, absorption properties and some aggregation reactions. M, Sc. Thesis University College; Dublin, Ireland.: