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Thromb Haemost 1976; 36(02): 311-318
DOI: 10.1055/s-0038-1648046
Original Article
Schattauer GmbH

Effects of Matrix Contact during Gel Filtration of Human Platelets in Plasma

Jack N. Lindon   *
1   Department of Biology, Massachusetts Institute of Technology, Cambridge, Mass, U.S.A.
,
Robert Rodvien   **
1   Department of Biology, Massachusetts Institute of Technology, Cambridge, Mass, U.S.A.
,
David F. Waugh
1   Department of Biology, Massachusetts Institute of Technology, Cambridge, Mass, U.S.A.
› Author Affiliations
Further Information

Publication History

Received 18 October 1975

Accepted 02 May 1976

Publication Date:
03 July 2018 (online)

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Summary

When gel filtration is used to transfer platelets from plasma into an established environment, alterations in platelet characteristics may result from the change in environment or from the effects of platelet contact with the gel matrix. To approach the problem of evaluating the relative contributions from these sources, a Sepharose 2B matrix was employed and platelets transferred from citrate anticoagulated PRP into autologous PPP to yield plasma-GFP. Platelet recoveries averaged 93 %. PRP: plasma-GFP pairs were found to be indistinguishable with respect to: morphology; ADP, thrombin or collagen-induced aggregation response; uptake of 5-hydroxytryptamine (5-HT) or adenosine; and thrombin or collagen-induced release of accumulated 5-HT or adenosine. Pairs are distinguishable by prostaglandin E2 synthesis assayed immediately after filtration.

* A portion of this work was submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy, Massachusetts Institute of Technology, 1974.


** Present address: Pacific Medical Center, San Francisco, California.


 

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