An immunological Method for Detection of the Carrier of Hemophilia B

Matsuzo Matsuoka; Masakazu Ito; Kaoru Takahashi; Nobuo Sakuragawa

doi:10.1055/s-0038-1648059

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1976; 36(02): 441-450
DOI: 10.1055/s-0038-1648059

Original Article

Schattauer GmbH

An immunological Method for Detection of the Carrier of Hemophilia B

Authors

Matsuzo Matsuoka

¹First Department of Internal Medicine, Niigata University School of Medicine, Niigata, Japan
Masakazu Ito

¹First Department of Internal Medicine, Niigata University School of Medicine, Niigata, Japan
Kaoru Takahashi

¹First Department of Internal Medicine, Niigata University School of Medicine, Niigata, Japan
Nobuo Sakuragawa

¹First Department of Internal Medicine, Niigata University School of Medicine, Niigata, Japan

Abstract

Summary

This paper presents the results of an immunological study of hemophilia B and its carriers which used two kinds of antibodies, a heterologous antibody of high specificity and a homologous antibody developed in a patient with severe hemophilia B.

1. The factor IX related antigen for hemophilia B could be determined by a neutralization test and not by a precipitation reaction.

2. The activity of factor IX in a definite carrier of hemophilia B was significantly lower than that of factor VIII in a definite carrier of hemophilia A.

3. In hemophilia B, there were no differences between the factor IX antigen determined by either the heterologous rabbit antibodies or the homologous antibodies. And there was no discrepancy between the factor IX antigen and the factor IX activity. Therefore, we cannot use this discrepancy for the detection of a carrier of hemophilia B.

4. However, there was a discrepancy between the factor IX antigen determined by the neutralization test and the factor IX procoagulant activity in the patients of both hemophilia B_M and hemophilia B⁺. The identical results were obtained in the cases of carriers of both hemophilia B_M and hemophilia B⁺. These facts are very useful for the detection of carriers of hemophilia B_M and hemophilia B⁺.

Full Text

References

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