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Thromb Haemost 1972; 27(01): 025-032
DOI: 10.1055/s-0038-1649006
Originalarbeiten — Original Articles — Travaux Originaux
Schattauer GmbH

Studies on Blood Coagulation Factor V

V. Changes of Molecular Weight Accompanying Activation of Factor V by Thrombin and the Procoagulant Protein of Russell’s Viper Venom
M. J. P Kahn
1   Laboratory for Coagulation Biochemistry and Cardiovascular Biochemical Research, Clinic for Internal Medicine, University Hospital, Leiden, The Netherlands
,
H. C Hemker
1   Laboratory for Coagulation Biochemistry and Cardiovascular Biochemical Research, Clinic for Internal Medicine, University Hospital, Leiden, The Netherlands
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Publication History

Publication Date:
29 June 2018 (online)

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Summary

It is confirmed that a protein from Russell’s viper venom has a direct activating action on human as well as bovine factor V.

Unactivated human factor V has a molecular weight as estimated by gel filtration of ∼410,000, after activation the molecular weight is ∼110,000, thus suggesting the dissociation of a tetramer.

The approximative molecular weight figures for unactivated and activated bovine factor V are 400,000 and 195,000, resp. This suggests the dissociation into a dimer accompanying the process of activation.

This article forms part of the Ph. D. thesis of the first author that was presented in March 1970 at the University of Leiden.


 

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