Use of the Megathrombocyte to Demonstrate Thrombopoietin

Weiner Marc; Karpatkin Simon

doi:10.1055/s-0038-1649038

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1972; 28(01): 024-030
DOI: 10.1055/s-0038-1649038

Original Article

Schattauer GmbH

Use of the Megathrombocyte to Demonstrate Thrombopoietin

Weiner Marc

¹Department of Medicine, New York University Medical School, New York, New York 10016

,

Karpatkin Simon

¹Department of Medicine, New York University Medical School, New York, New York 10016

› Author Affiliations

Abstract

Summary

Quantitation of megathrombocyte (large platelet) number was used as an assay for the detection of a humorally-transmitted thrombopoietic stimulus, thrombopoietin. Donor guinea pigs were depleted of circulating platelets by the injection of rabbit anti-guinea pig platelet antibody. Plasma from these donor guinea pigs, when injected into recipient guinea pigs raised their platelet count 1.5 fold and their megathrombocyte number 2.7 fold when compared to plasma injected from donor guinea pigs with normal platelet counts. A lag period of 4 to 5 days preceded the rise in platelet count and megathrombocyte number.

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References

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