Thromb Haemost 1972; 28(02): 221-227
DOI: 10.1055/s-0038-1649058
Original Article
Schattauer GmbH

The Effect of Neuraminidase on Platelet Aggregation Induced by ADP, Norepinephrine, Collagen or Serotonin

James W. Davis
1   Hematology Research Laboratories, Veterans Administration Hospital, Kansas City, Missouri and Departments of Medicine and Pathology, University of Kansas School of Medicine, Kansas City, Kansas
,
Kenneth T. N. Yue
1   Hematology Research Laboratories, Veterans Administration Hospital, Kansas City, Missouri and Departments of Medicine and Pathology, University of Kansas School of Medicine, Kansas City, Kansas
,
Phyllis E. Phillips
1   Hematology Research Laboratories, Veterans Administration Hospital, Kansas City, Missouri and Departments of Medicine and Pathology, University of Kansas School of Medicine, Kansas City, Kansas
› Author Affiliations
Further Information

Publication History

Publication Date:
29 June 2018 (online)

Zoom Image

Summary

Incubation of human platelet-rich plasma (PRP) with neuraminidase enhanced platelet aggregation induced by adenosine diphosphate (ADP), norepinephrine, collagen or serotonin. Both first and second phases of ADP- and norepinephrine -induced aggregation were enhanced. In two plasmas a second phase of ADP-induced aggregation occurred after incubation with neuraminidase, but not in the control preparations. In one plasma a second phase of serotonin-induced platelet aggregation occurred after incubation with neuraminidase. The incubation of PRP with exogenous N-acetylneuraminic acid (a product of the action of neuraminidase) had no effect on platelet aggregation. A possible explanation of the observed enhancement of platelet aggregation by neuraminidase is that the enzyme may have released sialic acid from platelet membranes and thereby reduced their net negative surface charge.